PMID: 1371424Jan 1, 1992

Studies of mineralization in tissue culture: optimal conditions for cartilage calcification

Bone and Mineral
A L BoskeyP Leboy

Abstract

The optimal conditions for obtaining a calcified cartilage matrix approximating that which exists in situ were established in a differentiating chick limb bud mesenchymal cell culture system. Using cells from stage 21-24 embryos in a micro-mass culture, at an optimal density of 0.5 million cells/20 microliters spot, the deposition of small crystals of hydroxyapatite on a collagenous matrix and matrix vesicles was detected by day 21 using X-ray diffraction, FT-IR microscopy, and electron microscopy. Optimal media, containing 1.1 mM Ca, 4 mM P, 25 micrograms/ml vitamin C, 0.3 mg/ml glutamine, no Hepes buffer, and 10% fetal bovine serum, produced matrix resembling the calcifying cartilage matrix of fetal chick long bones. Interestingly, higher concentrations of fetal bovine serum had an inhibitory effect on calcification. The cartilage phenotype was confirmed based on the cellular expression of cartilage collagen and proteoglycan mRNAs, the presence of type II and type X collagen, and cartilage type proteoglycan at the light microscopic level, and the presence of chondrocytes and matrix vesicles at the EM level. The system is proposed as a model for evaluating the events in cell mediated cartilage calcification.

References

Jun 1, 1996·The Anatomical Record·J E Davies
Jul 9, 1999·Journal of Biomedical Materials Research·S MoraisG S Carvalho
Oct 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C ReyM J Glimcher
Nov 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A L BoskeyI Binderman
Dec 1, 1992·Calcified Tissue International·A L BoskeyI Binderman
Jan 28, 2009·Calcified Tissue International·A L BoskeyMitchell B Schaffler
Mar 1, 1997·In Vitro Cellular & Developmental Biology. Animal·R A KandelM Speagle
May 15, 2002·Biochimica Et Biophysica Acta·Howard H T HsuFrancis Sun
Oct 22, 2003·Developmental Biology·Maria NurminskayaThomas F Linsenmayer
Sep 20, 2008·Chemical Reviews·A L Boskey, Rani Roy
Aug 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C ShukunamiY Hiraki
Apr 3, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B O OyajobiB M Stringer
Mar 1, 1996·Infection·M A MontD S Hungerford
Mar 20, 2013·British Journal of Clinical Pharmacology·Navid ShobeiriRachel M Holden
Aug 5, 2006·Journal of Cellular Biochemistry·Eric P PourmandA L Boskey
Jan 1, 1996·Connective Tissue Research·A L Boskey
Jun 11, 2011·Acta Orthopaedica·Darja Obradovic Wagner, Per Aspenberg
Sep 6, 2006·The Journal of Medical Investigation : JMI·Akemichi UenoTakafumi Noma
Sep 11, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Berivan CecenHasan Havitcioglu
Aug 26, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·E S TorresM E L Duarte
Dec 1, 1995·Matrix Biology : Journal of the International Society for Matrix Biology·M PinesA Bar
Feb 12, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A C TufanRocky S Tuan
Sep 25, 2003·Journal of Cellular Biochemistry·George R Beck
Jun 19, 2001·The Journal of Biological Chemistry·D WangJ E Henderson
Sep 7, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M A Mello, Rocky S Tuan
Nov 1, 1994·Journal of Cellular Biochemistry·B D BoyanZ Schwartz
Jul 1, 2010·Journal of Cellular Biochemistry·Rani RoyA L Boskey
Jun 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A C Tufan, R S Tuan
Mar 1, 1996·Calcified Tissue International·A L BoskeyI Binderman

Citations

Jul 1, 1976·Clinical Orthopaedics and Related Research·D S Howell, J C Pita
Apr 1, 1977·Journal of Ultrastructure Research·W J LandisM J Glimcher
Mar 13, 1979·Calcified Tissue International·N C BlumenthalL C Rosenberg
Oct 12, 1979·Science·I BindermanJ P Pennypacker
Apr 1, 1989·Developmental Biology·C TacchettiR Cancedda
Jan 1, 1989·Calcified Tissue International·R MendelsohnA L Boskey
Jan 1, 1989·Connective Tissue Research·I BindermanD Somjen
Nov 1, 1989·The Journal of Cell Biology·P BrucknerM A Spycher
Feb 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D F Paulsen, M Solursh
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S SaiM L Tanzer
Mar 1, 1974·The Journal of Experimental Zoology·M Solursh, S Meier
Jan 1, 1984·Calcified Tissue International·I R DicksonS S Jande
Jan 1, 1983·Metabolic Bone Disease & Related Research·H K VäänänenH C Anderson
Jan 1, 1982·Calcified Tissue International·H C Tenenbaum, J N Heersche
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·F SuzukiY Shimomura
Oct 6, 1980·Biochimica Et Biophysica Acta·C L ParkerL King
Jan 1, 1951·Journal of Morphology·V HAMBURGER, H L HAMILTON

Related Concepts

Calcium
Collagen Diseases
Extracellular Matrix
Aggrecans
Limb Structure
MATN1
Zyderm
X-Ray Diffraction
Neoglycoproteins
Electron Microscopy

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