Studies of minute quantities of natural abundance molecules using 2D heteronuclear correlation spectroscopy under 100 kHz MAS

Solid State Nuclear Magnetic Resonance
Yusuke NishiyamaMarek Pruski

Abstract

Two-dimensional (1)H{(13)C} heteronuclear correlation solid-state NMR spectra of naturally abundant solid materials are presented, acquired using the 0.75-mm magic angle spinning (MAS) probe at spinning rates up to 100 kHz. In spite of the miniscule sample volume (290 nL), high-quality HSQC-type spectra of bulk samples as well as surface-bound molecules can be obtained within hours of experimental time. The experiments are compared with those carried out at 40 kHz MAS using a 1.6-mm probe, which offered higher overall sensitivity due to a larger rotor volume. The benefits of ultrafast MAS in such experiments include superior resolution in (1)H dimension without resorting to (1)H-(1)H homonuclear RF decoupling, easy optimization, and applicability to mass-limited samples. The HMQC spectra of surface-bound species can be also acquired under 100 kHz MAS, although the dephasing of transverse magnetization has significant effect on the efficiency transfer under MAS alone.

References

Aug 1, 1995·Journal of Magnetic Resonance. Series B·T L PeckP C Lauterbur
Jan 5, 2000·Journal of Magnetic Resonance·B M FungK Ermolaev
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Chad M RienstraRobert G Griffin
Feb 5, 2003·Journal of Magnetic Resonance·B Reif, R G Griffin
Dec 18, 2003·Journal of the American Chemical Society·Matthias ErnstBeat H Meier
Apr 15, 2004·Journal of the American Chemical Society·Matthias ErnstBeat H Meier
Aug 30, 2007·Journal of the American Chemical Society·Donghua H ZhouChad M Rienstra
Dec 5, 2008·Journal of the American Chemical Society·Ségolène LaageLyndon Emsley
Dec 11, 2008·Journal of the American Chemical Society·Vikram S BajajRobert G Griffin
Feb 10, 2009·Nature Methods·Nalinda P WickramasingheYoshitaka Ishii
Jun 2, 2009·Journal of Magnetic Resonance·Michal LeskesShimon Vega
Jan 23, 2010·Journal of Magnetic Resonance·Kanmi Mao, Marek Pruski
Jun 24, 2010·Journal of Magnetic Resonance·Jean-Philippe DemersAdam Lange
Dec 2, 2011·Journal of Magnetic Resonance·Yusuke NishiyamaJean-Paul Amoureux
Mar 7, 2012·Topics in Current Chemistry·Guido Pintacuda, Gwendal Kervern
Sep 19, 2012·Journal of Magnetic Resonance·Xingyu LuJean-Paul Amoureux
Jun 4, 2013·Journal of Magnetic Resonance·Piotr PaluchMarek J Potrzebowski
Jan 16, 2014·Journal of Magnetic Resonance·Yue Qi YeYusuke Nishiyama

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Citations

Jan 24, 2016·The Journal of Chemical Physics·Rongchun Zhang, Ayyalusamy Ramamoorthy
Oct 17, 2015·The Journal of Chemical Physics·Rongchun ZhangAyyalusamy Ramamoorthy
Jul 12, 2016·Solid State Nuclear Magnetic Resonance·Yusuke Nishiyama
Mar 4, 2017·Acta Crystallographica. Section C, Structural Chemistry·Tetsuo OikawaYusuke Nishiyama
Apr 2, 2016·Physical Chemistry Chemical Physics : PCCP·Sachin R ChaudhariLyndon Emsley
Jun 18, 2016·Physical Chemistry Chemical Physics : PCCP·Stanislav L VeinbergRobert W Schurko
Oct 5, 2018·Physical Chemistry Chemical Physics : PCCP·Nghia Tuan DuongJean-Paul Amoureux
Aug 3, 2018·The Journal of Chemical Physics·Sharon E Ashbrook, Paul Hodgkinson
Aug 8, 2019·Nature Communications·Candelaria Guzmán-AfonsoYusuke Nishiyama
Apr 11, 2017·Solid State Nuclear Magnetic Resonance·Amrit VenkateshAaron J Rossini
Jun 22, 2021·Solid State Nuclear Magnetic Resonance·Takeshi KobayashiYusuke Nishiyama
May 26, 2017·The Journal of Physical Chemistry. B·Rongchun ZhangAyyalusamy Ramamoorthy

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