PMID: 4907882Nov 1, 1969Paper

Studies of newly synthesized ribosomal ribonucleic acid of Escherichia coli

The Biochemical Journal
M Fry, M Artman

Abstract

1. RNA synthesized by Escherichia coli during one-hundredth of the generation time contains two fractions distinguishable by hybridization with homologous DNA. One fraction, approximately 30% of the newly synthesized RNA, did not compete with ribosomal RNA, being apparently messenger RNA. The other fraction, approximately 70% of the newly made RNA, hybridized as ribosomal RNA. These values are comparable with previous estimates (McCarthy & Bolton, 1964; Pigott & Midgley, 1968). 2. Hybridization-competition experiments showed that the newly made RNA associated with 70s ribosomes and larger ribosome aggregates was a mixture of ribosomal RNA and messenger RNA, whereas that associated with nascent ribosomal subunits consisted exclusively of ribosomal RNA. This observation provides means by which newly synthesized ribosomal RNA can be isolated free from messenger RNA. 3. Newly made ribosomal RNA in nascent ribosomal subunits was sensitive to shear under conditions where ribosomal RNA in mature ribosomes was shear-resistant. Thus, when RNA was extracted from cells of E. coli disrupted by mechanical means, newly made ribosomal RNA appeared heterogeneous in size, sedimenting as a broad peak extending from 8s to 16s. 4. Newly synthesize...Continue Reading

Citations

Dec 1, 1970·Archives of Biochemistry and Biophysics·M S van Dijk-Salkinoja, R J Planta
Sep 1, 1970·Bacteriological Reviews·M Nomura
Dec 30, 1972·Journal of Molecular Biology·P Donini
Jul 10, 1974·Biochemical and Biophysical Research Communications·M Artman, S Werthamer

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