PMID: 479917Jul 1, 1979

Studies of nuclear proteins in the skeletal muscle of the cardiomyopathic Syrian hamster

Journal of the Neurological Sciences
I H FraserC C Liew


Nonhistone nuclear proteins (NHNP) were isolated from the skeletal muscle of dystrophic hamsters and their respective paired controls. These proteins were extracted with phenol and fractionated by polyacrylamide gel electrophoresis. One-dimensional gel electrophoresis using either sodium-dodecylsulfate or isoelectrofocusing revealed quantitative changes between the two groups. An improved resolution using a two-dimensional system showed both quantitative and very limited qualitative differences. There are differences in proteins focusing at pH 5.0, molecular weight 55,000, in the dystrophic tissue. In addition proteins from dystrophic muscle focusing between pH 5.0 and 7.0, molecular weight 45,000, and proteins focusing between pH 7.0 and 9.0, molecular weights 35,000--45,000 and 60,000--70,000 were reduced as compared to the controls. There were no detectable differences in the electrophoretic patterns between the two groups of proteins derived from skeletal muscle homogenates. The differences in NHNP appear to be reflections of alterations in the nuclear composition of the dystrophic muscle cell. Some of these differences may represent changes secondary to the muscle disease. However, if NHNP interacting with DNA play a major...Continue Reading


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Mar 1, 1974·Science·G S SteinL J Kleinsmith
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Cell Fractionation
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