PMID: 479917Jul 1, 1979

Studies of nuclear proteins in the skeletal muscle of the cardiomyopathic Syrian hamster

Journal of the Neurological Sciences
I H FraserC C Liew

Abstract

Nonhistone nuclear proteins (NHNP) were isolated from the skeletal muscle of dystrophic hamsters and their respective paired controls. These proteins were extracted with phenol and fractionated by polyacrylamide gel electrophoresis. One-dimensional gel electrophoresis using either sodium-dodecylsulfate or isoelectrofocusing revealed quantitative changes between the two groups. An improved resolution using a two-dimensional system showed both quantitative and very limited qualitative differences. There are differences in proteins focusing at pH 5.0, molecular weight 55,000, in the dystrophic tissue. In addition proteins from dystrophic muscle focusing between pH 5.0 and 7.0, molecular weight 45,000, and proteins focusing between pH 7.0 and 9.0, molecular weights 35,000--45,000 and 60,000--70,000 were reduced as compared to the controls. There were no detectable differences in the electrophoretic patterns between the two groups of proteins derived from skeletal muscle homogenates. The differences in NHNP appear to be reflections of alterations in the nuclear composition of the dystrophic muscle cell. Some of these differences may represent changes secondary to the muscle disease. However, if NHNP interacting with DNA play a major...Continue Reading

References

Jan 1, 1976·Canadian Journal of Biochemistry·G JackowskiC C Liew
Mar 15, 1975·Experimental Cell Research·L Kuehl
Mar 1, 1977·Experimental Cell Research·I R HeldH Tonaki
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·C C Liew, P K Chan
Nov 1, 1965·The Journal of Cell Biology·J C EdelmanI L Schwartz
Mar 1, 1974·Science·G S SteinL J Kleinsmith
Oct 23, 1974·Biochemical and Biophysical Research Communications·C B ChaeH P Morris
Apr 25, 1972·Biochemistry·S LevyH A Sober
Apr 1, 1970·Archives of Biochemistry and Biophysics·B E Sobel, S Kaufman
Jul 14, 1968·Journal of Molecular Biology·J Paul, R S Gilmour

Citations

Jun 1, 1981·Journal of the Neurological Sciences·S OkuC C Liew
Jan 1, 1982·Critical Reviews in Clinical Laboratory Sciences·C S Giometti

Related Concepts

Metazoa
Cell Fractionation
Cell Nucleus
Chromosomal Proteins, Non-Histone
SDS-PAGE
Cricetus
Isoelectric Focusing
Mesocricetus
Electron Microscopy
Muscle

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