Studies of pigment transfer between Xenopus laevis melanophores and fibroblasts in vitro and in vivo

Pigment Cell Research
Sara AspengrenMargareta Wallin

Abstract

Frog melanophores rapidly change colour by dispersion or aggregation of melanosomes. A long-term colour change exists where melanosomes are released from melanophores and transferred to surrounding skin cells. No in vitro model for pigment transfer exists for lower vertebrates. Frog melanophores of different morphology exist both in epidermis where keratinocytes are present and in dermis where fibroblasts dominate. We have examined whether release and transfer of melanosomes can be studied in a melanophore-fibroblast co-culture, as no frog keratinocyte cell line exists. Xenopus laevis melanophores are normally cultured in conditioned medium from fibroblasts and fibroblast-derived factors may be important for melanophore morphology. Melanin was exocytosed as membrane-enclosed melanosomes in a process that was upregulated by alpha-melanocyte-stimulating hormone (alpha-MSH), and melanosomes where taken up by fibroblasts. Melanosome membrane-proteins seemed to be of importance, as the cluster-like uptake pattern of pigment granules was distinct from that of latex beads. In vivo results confirmed the ability of dermal fibroblasts to engulf melanosomes. Our results show that cultured frog melanophores can not only be used for studies...Continue Reading

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Citations

Oct 22, 2008·Seminars in Cell & Developmental Biology·Cynthia D Cooper, David W Raible
Aug 8, 2009·Experimental Dermatology·P M PlonkaK U Schallreuter
Jun 22, 2006·Traffic·Karolien Van Den BosscheJo Lambert
Jun 22, 2012·Experimental Dermatology·Agnieszka Wolnicka-GlubiszPrzemyslaw M Plonka
Jan 6, 2009·International Review of Cell and Molecular Biology·Sara AspengrenMargareta Wallin
Sep 27, 2018·Physiological Reviews·Liliana D'Alba, Matthew D Shawkey
Dec 7, 2006·Journal of Neuroscience Research·Sara AspengrenMargareta Wallin
Mar 12, 2020·Molecular Ecology·James D BurgonKathryn R Elmer
Aug 19, 2020·International Journal of Molecular Sciences·Hideya AndoMasamitsu Ichihashi
Mar 5, 2021·The Australasian Journal of Dermatology·Anuradha BishnoiMuthu Sendhil Kumaran
May 1, 2021·International Journal of Molecular Sciences·Hugo MoreirasDuarte C Barral

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