PMID: 6160471Aug 11, 1980Paper

Studies of RNA release reaction catalyzed by E. coli transcription termination factor rho using isolated ternary transcription complexes

Nucleic Acids Research
K Shigesada, C W Wu

Abstract

Protein factor rho catalyzes site-specific termination of transcription in a reaction requiring hydrolysis of nucleoside triphosphate with eventual release of RNA from RNA polymerase and DNA template. We have characterized the rho-catalyzed RNA release reaction using isolated transcription complexes. Transcription complexes containing T7 D111 DNA, RNA polymerase, and 3H-labeled nascent RNA were formed and isolated by gel filtration on an Agarose 5M column. When the ternary complexes were incubated with rho factor in the presence of ATP, or dATP, significant amounts of nascet RNA were released from the complexes as determined in a membrane filtration assay. Gel electrophoretic analysis of RNA has revealed that rho releases selected species of discrete-sized RNA from among those originally present in the ternary complexes. These results show that rho essentially acts to release RNA from those ternary complexes which have come to pause, and that this reaction proceeds in a discrete step separately from the pausing of RNA synthesis. Under the conditions used, the extent of RNA release widely varied at individual pausing sites and thus the action of rho exhibited certain site-selectivity.

References

Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A M Wu, T Platt
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Feb 28, 1973·Nature: New Biology·B De CrombruggheI Pastan
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C Lowery-Goldhammer, J P Richardson
Aug 7, 1972·Biochemical and Biophysical Research Communications·J HurwitzS Wickner
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·N M Maizels
Jun 25, 1973·Journal of Molecular Biology·E G Minkley, D Pribnow
Nov 1, 1969·Virology·F W Studier
Dec 20, 1969·Nature·J W Roberts

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Citations

Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R B Kent, S K Guterman
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A J Dombroski, T Platt
May 24, 1985·Nucleic Acids Research·W D MorganP H von Hippel
Sep 25, 1981·Nucleic Acids Research·K IneichenT A Bickle
Jun 16, 2006·RNA·Maxim V SukhodoletsSankar Adhya
Aug 27, 1981·Biochimica Et Biophysica Acta·S L Bektesh, J P Richardson
May 17, 2008·The Journal of Biological Chemistry·Dipak DuttaRanjan Sen
Jul 22, 2017·Annual Review of Microbiology·Pallabi MitraRanjan Sen
Apr 17, 1999·The Journal of Biological Chemistry·D E KimS S Patel
Sep 6, 2002·Biochimica Et Biophysica Acta·John P Richardson
Apr 6, 1990·Biochimica Et Biophysica Acta·J P Richardson

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