Studies of selenium and arsenic mutual protection in human HepG2 cells

Chemico-biological Interactions
Gurnit KaurElaine M Leslie

Abstract

Hundreds of millions of people worldwide are exposed to unacceptable levels of carcinogenic inorganic arsenic. Animal models have shown that selenium and arsenic are mutually protective through the formation and elimination of the seleno-bis(S-glutathionyl) arsinium ion [(GS)2AsSe]-. Consistent with this, human selenium deficiency in arsenic-endemic regions is associated with arsenic-induced disease, leading to the initiation of human selenium supplementation trials. In contrast to the protective effect observed in vivo, in vitro studies have suggested that selenite increases arsenite cellular retention and toxicity. This difference might be explained by the rapid conversion of selenite to selenide in vivo. In the current study, selenite did not protect the human hepatoma (HepG2) cell line against the toxicity of arsenite at equimolar concentrations, however selenide increased the IC50 by 2.3-fold. Cytotoxicity assays of arsenite + selenite and arsenite + selenide at different molar ratios revealed higher overall mutual antagonism of arsenite + selenide toxicity than arsenite + selenite. Despite this protective effect, in comparison to 75Se-selenite, HepG2 cells in suspension were at least 3-fold more efficient at accumulating ...Continue Reading

References

Aug 1, 1977·Environmental Health Perspectives·O A Levander
Jan 1, 1973·Acta Pharmacologica Et Toxicologica·M Sandholm
Jul 1, 1966·Toxicology and Applied Pharmacology·O A Levander, C A Baumann
Aug 1, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·Y Shiobara, K T Suzuki
Nov 20, 2002·Chemical Research in Toxicology·Jürgen GailerJ J Winzerling
Feb 27, 2003·Toxicology·Iván Csanaky, Zoltán Gregus
Apr 26, 2003·Biological Trace Element Research·Amanda S NiskarMichael McGeehin
Aug 4, 2004·Free Radical Biology & Medicine·Tom K Hei, Metka Filipic
Apr 13, 2006·Chemical Research in Toxicology·Shawn A ManleyJürgen Gailer
Jul 18, 2006·Chemical Research in Toxicology·Zuzana DrobnaMiroslav Stýblo
Aug 29, 2006·Toxicology·Lucia LetavayováJela Brozmanová
Feb 16, 2007·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Yu ChenHabibul Ahsan
May 19, 2007·Antioxidants & Redox Signaling·Laura Vanda PappKum Kum Khanna
Aug 10, 2007·Journal of Agricultural and Food Chemistry·Dil ThavarajahIngrid J Pickering
Nov 22, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Zhi HuangXinrong Zhang
Dec 25, 2007·FEBS Letters·Dennis Ganyc, William T Self
Jun 14, 2008·Biology of the Cell·Catherine DecaensDoris Cassio
Jun 20, 2008·Inorganic Chemistry·Mamoru HaratakeMorio Nakayama
Jun 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Eric OlmMikael Björnstedt
Jan 6, 2011·Environmental Health Perspectives·J Richard PilsnerMary V Gamble
Jun 15, 2011·Bioscience, Biotechnology, and Biochemistry·Takashi TamuraKenji Inagaki
Sep 16, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Michael W CarewElaine M Leslie
Feb 24, 2012·Metallomics : Integrated Biometal Science·Kanwal Rehman, Hua Naranmandura
Apr 13, 2012·The Journal of Experimental Biology·Sougat MisraSom Niyogi
Oct 16, 2012·Nature Biotechnology·Xu TanStephen J Elledge
Mar 6, 2013·Environmental Health Perspectives·Marisa F NaujokasWilliam A Suk
Jun 27, 2013·Toxicology and Applied Pharmacology·Shweta SahJudit Smits
Jul 3, 2013·Chemical Reviews·Shengwen ShenX Chris Le
Jul 3, 2013·Environmental Health : a Global Access Science Source·Christine Marie GeorgeJoseph Graziano
Nov 5, 2013·Metallomics : Integrated Biometal Science·Marco RomanCarlo Barbante
Jan 29, 2014·Proceedings of the National Academy of Sciences of the United States of America·Lesley A Mathews GrinerCraig J Thomas
Feb 13, 2014·Chemical Research in Toxicology·William R Cullen
May 24, 2014·Environment International·Hong-Jie SunLena Q Ma
Aug 8, 2014·Chemical Reviews·M Jake PushieGraham N George
Nov 19, 2014·Chemical Research in Toxicology·Lenora K HarperCraig A Bayse
Apr 4, 2015·Nutrients·Gerald F Combs

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.