Studies of temperature-sensitive transfer and maintenance of H incompatibility group plasmids

Journal of General Microbiology
Diane E Taylor, J G Levine

Abstract

The mechanism of temperature-sensitive transfer was studied for plasmids of the H incompatibility group. Transfer depended on the temperature of the mating mixture but the growth temperature of the donor was also important, and donor cells previously grown at 26 degrees C could not facilitate transfer at 37 degrees C. Comparison of transfer characteristics of a non-thermosensitive H plasmid R831b and thermosensitive H plasmids from Salmonella from Ontario during a 2 h mating period showed that the thermosensitive phenotype inhibited transfer by about 200-fold at 37 degrees C and by 10-fold at 42 degrees C. At temperatures between 15 and 30 degrees C, the thermotolerant H plasmid transferred at about the same frequency as the temperature-sensitive plasmid. Elimination of some H plasmids after growth of host cells was also observed and physical evidence of this was obtained. The characteristic of high-temperature elimination (Hte) was limited to plasmids from similar bacterial and geographphical sources. Plasmids from Salmonella spp. isolated in Ontario did not possess this phenotype, whereas plasmids from Serratia marcescens isolated in the United States did. Although the Tra(ts) and Hte phenotypes may both be characteristic of ...Continue Reading

Citations

Feb 12, 2013·Plasmid·Marta GibertCarlos Balsalobre
Apr 19, 2012·The Journal of Antimicrobial Chemotherapy·Laura VillaAlessandra Carattoli
Aug 14, 2012·The Journal of Antimicrobial Chemotherapy·Amy K Cain, Ruth M Hall
Oct 23, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cynthia Portal-CelhayMartin J Blaser
May 22, 2009·Antimicrobial Agents and Chemotherapy·Diane E Taylor
May 1, 1981·Antimicrobial Agents and Chemotherapy·D E TaylorP C Fleming
Oct 24, 2014·Emerging Infectious Diseases·Nicholas A FeaseyRobert A Kingsley
Feb 16, 2011·Research in Microbiology·Rosa C BañosAntonio Juárez
Jan 15, 2008·Plasmid·James E GuntonDiane E Taylor
Feb 28, 2009·Advances in Applied Microbiology·Wolfgang Schumann
Jan 27, 2016·PloS One·María GetinoFernando de la Cruz
Feb 1, 1994·Molecular Microbiology·P J Newnham, D E Taylor
Jan 1, 1997·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·A FicaF Cabello
Jun 10, 2020·Antimicrobial Agents and Chemotherapy·Gregory H TysonShaohua Zhao
Aug 6, 2020·Frontiers in Molecular Biosciences·Marta GibertCarlos Balsalobre

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