PMID: 207346May 25, 1978

Studies of the cyanogen bromide fragments of the apoprotein of human serum low density lipoproteins

Biochimica Et Biophysica Acta
D G DeutschA M Scanu

Abstract

The apoprotein of human serum low density lipoproteins was reduced and carboxymethylated and then cleaved by cyanogen bromide (CNBr). The peptides which were produced from this cleavage (90% yield, based upon loss of methionine) were resolved by SDS polyacrylamide gel electrophoresis into 10 major bands, each having an amino acid composition very similar to that of intact reduced and carboxymethylated LDL apoprotein. The fractionation of the CNBr fragments by preparative gel filtration was dependent upon the nature of the eluting solvent. NH4OH and SDS solvents eluted all of the material in the void volume. In 6 M guanidinium chloride solvents several peaks were, however, resolved, each having an amino acid composition similar to that of the unfractionated products. Whereas no NH2-terminal was detected in reduced and carboxylmethylated LDL apoprotein, automated Edman degradation of the protein following treatment with CNBr revealed the presence of several NH2-termini. The results suggest that LDL apoprotein may be made of segments of, at least, very similar amino acid composition and that both the protein itself and derivative fragments have a great tendency to aggregate even in denaturing solvents.

References

Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B W ShenF J Kézdy
Mar 1, 1974·CRC Critical Reviews in Biochemistry·A M ScanuS Deganello
Apr 1, 1974·FEBS Letters·K V Krishnaiah, H Wiegandt
Aug 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J P KaneR J Havel
Sep 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·H PollardE W Taylor
Jun 28, 1968·Annals of the New York Academy of Sciences·F A Anderer, S Hörnle
Mar 1, 1970·Journal of Molecular Biology·A V Crewe, J Wall
Sep 27, 1967·Biochemical and Biophysical Research Communications·B Shore, V Shore
Dec 15, 1996·Circulation·S SchäferB E Strauer

Citations

Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T S Watt, R M Watt
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L S HuangJ L Breslow
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C F WeiL Chan
Mar 14, 2002·Biochemical and Biophysical Research Communications·Ananth Sekher PannalaCatherine A Rice-Evans
Nov 1, 1980·European Journal of Biochemistry·D R van der WesthuyzenG A Coetzee

Related Concepts

Apolipoproteins
Molecular Sieve Chromatography
Cyanogen Bromide
LDL-1
Oxidation-Reduction
Peptide Fragments
Polypeptides

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.