PMID: 6406421Apr 1, 1983

Studies of the extracellular proteolytic activity produced by Propionibacterium acnes

The Journal of Applied Bacteriology
E IngramW J Cunliffe

Abstract

Of several commercial media tested, trypticase soya both containing 0.4% (w/v) D-sorbitol was superior as a growth medium for the production of extracellular proteinase by Propionibacterium acnes (strain P-37). Extracellular proteinase, production of which was shown to be growth-associated by both batch and continuous culture studies, was partially purified by 70% (NH4)2SO4 saturation. Sephadex G-75 chromatography and ion exchange on DEAE-Sephadex A-50. It was shown to be a heterogeneous mixture of at least three molecular species of enzyme. Proteinase I was inhibited by EDTA (10(-3) mol/l) and PMSF (5 millimol/l) and stimulated by CaCl2 (190% at 10(-3) mol/l). It had a molecular weight of 20 to 30000 and a broad pH optimum from 6.5 to 7.5. Proteinase II was an alkaline proteinase with a molecular weight of 30 to 40000 which was not significantly inhibited by EDTA (10(-2) mol/l) nor stimulated by CaCl2. Proteinase III represented a minor proportion of the recovered proteolytic activity, had a molecular weight of 20 to 30000 and was most active in the alkaline pH range. This enzyme was inhibited by EDTA (10(-4) mol/l) and PMSF (5 millimol/l), and stimulated by CaCl2 (250% at 10(-2) mol/l).

References

Jun 1, 1977·Journal of Bacteriology·A T AbdelalD G Ahearn
Dec 1, 1979·The Journal of Applied Bacteriology·K T HollandW J Cunliffe
Jan 1, 1978·Applied and Environmental Microbiology·K J McGinleyJ J Leyden
Jan 1, 1976·Annual Review of Microbiology·A R Glenn
Aug 1, 1974·Journal of Medical Microbiology·R R Marples, K J McGinley
Sep 1, 1981·The Journal of Investigative Dermatology·R M LavkerK J McGinley
Oct 1, 1981·The Journal of Applied Bacteriology·K T HollandW J Cunliffe
Jun 1, 1981·Journal of General Microbiology·E InghamW J Cunliffe
Mar 20, 1947·The Journal of General Physiology·M Kunitz

Citations

May 1, 1994·World Journal of Microbiology & Biotechnology·N A RazakA B Salleh
Dec 12, 2007·Bioprocess and Biosystems Engineering·S ShanmughapriyaK Natarajaseenivasan
Jan 17, 2004·Microbial Pathogenesis·Zsuzsanna CsukásFerenc Rozgonyi
Jun 29, 2002·Journal of Dermatological Science·QiJie ChenMasutaka Furue
May 14, 2014·The Journal of Investigative Dermatology·Zheng Jun LiMyung Im
Feb 7, 2013·The British Journal of Dermatology·F DalgardG Yosipovitch

Related Concepts

Calcium Chloride, Anhydrous
Edetic Acid, Sodium Salt
Hydrogen-Ion Concentration
Endopeptidases
Phenylmethylsulfonyl Fluoride
Propionibacterium
Propionibacterium acnes

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