PMID: 9525665Apr 3, 1998Paper

Studies of the nucleopolyhedrovirus infection process in insects by using the green fluorescence protein as a reporter

Journal of Virology
J W BarrettS R Palli

Abstract

A recombinant Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) expressing the green fluorescence protein (GFP) under the control of the AcMNPV polyhedrin promoter was constructed to study the spatial and temporal regulation of baculovirus infection in a permissive host. Larvae that ingested AcMNPV-GFP showed localized expression of GFP in the midgut epithelial cells, as well as hemocytes, at 24 h postinfection. The presence of fluorescence in these tissues indicated not only that the virus was replicating but also that the very late viral proteins were being synthesized. Secondary infection occurred within the tracheal cells throughout the body cavity, confirming earlier reports, and these foci of infection allowed entry of the virus into other tissues, such as the epidermis and the fat body.

References

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Citations

Jun 10, 2004·Journal of Medical Entomology·William S RomoserGeorge V Ludwig
Jun 14, 2012·Archives of Virology·Tamer Z SalemXiao-Wen Cheng
Jan 22, 2011·Current Microbiology·Guohui LiKeping Chen
Mar 5, 2016·Zoological Letters·Bidur DhungelJoji M Otaki
Mar 3, 2011·Genetics and Molecular Research : GMR·L A DouradoM A Fernandez
Feb 18, 2019·Journal of Economic Entomology·Sóstenez A Vessaro-SilvaCamila Maria Toigo de Oliveira
Apr 27, 2007·Virology·Chanitchote DetvisitsakunA Lorena Passarelli

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