Studies of the RNA degradosome-organizing domain of the Escherichia coli ribonuclease RNase E

Journal of Molecular Biology
Anastasia J CallaghanBen F Luisi

Abstract

The hydrolytic endoribonuclease RNase E, which is widely distributed in bacteria and plants, plays key roles in mRNA degradation and RNA processing in Escherichia coli. The enzymatic activity of RNase E is contained within the conserved amino-terminal half of the 118 kDa protein, and the carboxy-terminal half organizes the RNA degradosome, a multi-enzyme complex that degrades mRNA co-operatively and processes ribosomal and other RNA. The study described herein demonstrates that the carboxy-terminal domain of RNase E has little structure under native conditions and is unlikely to be extensively folded within the degradosome. However, three isolated segments of 10-40 residues, and a larger fourth segment of 80 residues, are predicted to be regions of increased structural propensity. The larger of these segments appears to be a protein-RNA interaction site while the other segments possibly correspond to sites of self-recognition and interaction with the other degradosome proteins. The carboxy-terminal domain of RNase E may thus act as a flexible tether of the degradosome components. The implications of these and other observations for the organization of the RNA degradosome are discussed.

References

Jan 1, 1975·Methods in Enzymology·T G Spring, F Wold
May 24, 1991·Science·A LupasJ Stock
Nov 3, 1995·The Journal of Biological Chemistry·L TarasevicieneB E Uhlin
Nov 1, 1994·Molecular Microbiology·B PyC F Higgins
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·A MiczakS Lin-Chao
Mar 1, 1997·Molecular Microbiology·S N Cohen, K J McDowall
Feb 12, 1998·Molecular Microbiology·E BlumC F Higgins
Jun 11, 1999·Biochemical and Biophysical Research Communications·M WachiK Nagai
Jun 17, 1999·FEMS Microbiology Reviews·R Rauhut, G Klug
Aug 16, 2000·RNA·D A Steege
Nov 28, 2000·Proteins·P RomeroA K Dunker
May 31, 2001·Journal of Molecular Graphics & Modelling·A K DunkerZ Obradovic
Oct 9, 2001·Bioinformatics·L Goodstadt, C P Ponting
Oct 26, 2001·Journal of Molecular Biology·K Kühnel, B F Luisi
Nov 20, 2001·Nucleic Acids Research·S JägerG Klug
Mar 29, 2002·The Journal of Biological Chemistry·Alexis A Diwa, Joel G Belasco
Apr 4, 2002·Molecular Microbiology·Chaitanya JainJoel G Belasco
May 10, 2002·Genes & Development·Maria C Ow, Sidney R Kushner
Aug 10, 2002·Journal of Bacteriology·Sidney R Kushner
Nov 8, 2002·Molecular Microbiology·Alexis A DiwaJoel G Belasco
Jun 26, 2003·Nucleic Acids Research·Rune LindingToby J Gibson
Sep 17, 2003·Genes & Development·Eric MasséSusan Gottesman
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jonathan A BernsteinSue Lin-Chao
Apr 2, 2004·The Journal of Biological Chemistry·Charlotte L Hanson, Carol V Robinson

❮ Previous
Next ❯

Citations

Jun 1, 2006·Biochemistry·Jiangang LiuA Keith Dunker
May 10, 2007·Journal of Proteome Research·Vladimir VacicA Keith Dunker
Jul 15, 2009·Cell Research·Bo HeA Keith Dunker
Apr 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Oleg N MurashkoSue Lin-Chao
Feb 18, 2011·The Journal of Biological Chemistry·Salima NurmohamedBen F Luisi
Sep 21, 2013·The Journal of Biological Chemistry·Zbigniew PietrasBen F Luisi
Mar 14, 2008·The Journal of Biological Chemistry·Aziz Taghbalout, Lawrence Rothfield
Jan 20, 2007·DNA and Cell Biology·Erin Haag BreeseMaureen A Harrington
Oct 19, 2010·Nucleic Acids Research·Steven W HardwickBen F Luisi
Aug 28, 2012·Nucleic Acids Research·Yi-Chun TsaiBen F Luisi
Sep 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Salima NurmohamedBen F Luisi
Jan 1, 2013·Journal of Bacteriology·Masaru TamuraStanley N Cohen
Jun 15, 2007·Journal of Virology·Sinu P JohnColleen B Jonsson
Apr 24, 2007·Annual Review of Microbiology·Agamemnon J Carpousis
Oct 10, 2008·BMC Genomics·A Keith DunkerVladimir N Uversky
Mar 7, 2014·PloS One·Daeyoung KimYong-Hak Kim
Dec 15, 2010·International Journal of Molecular Sciences·Bin XueVladimir N Uversky
Nov 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Pei-Hsun Lin, Sue Lin-Chao
Feb 19, 2016·Current Microbiology·Daeyoung KimKangseok Lee
Dec 30, 2014·Journal of Molecular Biology·Tatsuaki KurataJun-ichi Kato
Feb 2, 2010·Biochimica Et Biophysica Acta·Vladimir N Uversky, A Keith Dunker
Apr 7, 2009·Progress in Biophysics and Molecular Biology·Jörg Gsponer, M Madan Babu
Nov 4, 2008·Molecular Microbiology·Vanessa KhemiciAgamemnon J Carpousis
Jul 21, 2006·Proteins·François FerronDavid Karlin
Dec 4, 2010·The FEBS Journal·Melissa A ErceMarc R Wilkins
Dec 13, 2012·Protein Science : a Publication of the Protein Society·Wei-Lun HsuA Keith Dunker
May 10, 2012·Molecular Microbiology·Martin Lehnik-HabrinkJörg Stülke
Nov 15, 2011·Molecular Microbiology·Marie Bouvier, Agamemnon J Carpousis
Nov 27, 2014·Protein Science : a Publication of the Protein Society·Patrick T DolanDouglas J LaCount
May 28, 2010·Protein Science : a Publication of the Protein Society·Adilia DagkessamanskaiaHélène Martin-Yken
Oct 7, 2004·Current Opinion in Structural Biology·Clay BrackenA Keith Dunker
Aug 29, 2006·Journal of Molecular Biology·Amrita MohanVladimir N Uversky
Aug 1, 2006·Trends in Biotechnology·Yugong ChengA Keith Dunker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.