Studies of various elements of nutritional and toxicological interest associated with different molecular weight fractions in Brazil nuts

Journal of Agricultural and Food Chemistry
Sasi S KannamkumarathJoseph A Caruso

Abstract

On-line hyphenation of size exclusion chromatography (SEC), UV, and inductively coupled plasma mass spectrometry (ICP-MS) was used to study the molecular weight distribution patterns of several elements in Brazil nuts (Bertholletia excelsa). This technique was used for the elemental speciation of different elements of nutritional and toxicological interests such as Mg, Fe, Co, Mo, Ag, Hg, and Pb. Elemental fractionation in Brazil nuts was studied using a Superdex peptide column with resolving capacity in the range of 14 to 0.18 kDa. Three different mobile phases, Tris buffer solution (pH 8.0), phosphate buffer (pH 7.5), and CAPS buffer solution (pH 10.0), were tried for the SEC fractionation. Size exclusion fractionation of all the extracted solutions was performed using a 50 mmol L(-)(1) Tris buffer (pH 8) as the mobile phase at a flow rate of 0.6 mL min(-)(1). Three different extractions, 0.05 mol L(-)(1) NaOH, 0.05 mol L(-)(1) HCl, and hot water at 60 degrees C, were performed, and the association of elements with various molecular weight fractions was evaluated. Total elemental concentrations in the extracted samples were determined and compared with the values obtained after total digestion to calculate the recovery values...Continue Reading

References

May 1, 1987·The American Journal of Clinical Nutrition·A D Mooradian, J E Morley
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May 16, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Richard KoplíkMiloslav Suchánek
Jul 13, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Richard KoplíkMiloslav Suchánek
Aug 13, 2002·Analytical and Bioanalytical Chemistry·Sasi S KannamkumarathJoseph A Caruso
Sep 19, 2002·Journal of Agricultural and Food Chemistry·Anne P VonderheideJoseph A Caruso

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Citations

Jul 30, 2014·Analytica Chimica Acta·Maria José da SilvaMiguel de la Guardia
Jan 11, 2014·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Maja Welna, Anna Szymczycha-Madeja
Jul 25, 2014·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Burcu KafaoğluDerya Kara

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