PMID: 6160922Aug 1, 1980Paper

Studies on a kallikrein-kinin system in plasma of patients with acute pancreatitis: the preparation and characterization of a kallikrein-like enzyme in patient's plasma

Clinical Biochemistry
S Takasugi, N Toki

Abstract

In a previous paper, the authors reported that the kallikrein-like activity was eluted in alpha 2-macroglobulin fractions when patient's plasma was fractionated with Sephadex G-200 gel filtration. In order to clarify the characteristics of the kallikrein-like enzyme, enzyme isolation methods were investigated. Success was attained by the addition of 1 % sodium-dodecyl-sulfate to alpha 2-macroglobulin fractions followed by G-200 chromatography. It was also confirmed that alpha 2-macroglobulin from which the enzyme was removed regained antiplasmin activity, and that some proteases other than the kallikrein-like enzyme were also bound to alpha 2-macroglobulin. The kallikrein-like enzyme isolated by sodium-dodecyl-sulfate-treatment was examined in respect of its molecular weight and its ability to be adsorbed on DEAE-cellulose and was found to possess a molecular weight approximating that of ovalbumin or human pancreatic kallikrein and a binding affinity for DEAE-cellulose. From these results, the authors speculate that during attacks of acute pancreatitis, the pancreas liberates kallikrein into the blood.

References

Jul 1, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·H SumiN Toki
Aug 1, 1970·The Journal of Experimental Medicine·P C Harpel
Mar 1, 1974·The British Journal of Surgery·J E TrapnellE H Duncan
Sep 7, 1967·Biochemical and Biophysical Research Communications·A L ShapiroJ V Maizel
Nov 1, 1958·The Journal of Experimental Medicine·P S NORMAN, B M HILL
May 1, 1961·Proceedings of the Society for Experimental Biology and Medicine·M E WEBSTER, J V PIERCE

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Citations

Nov 30, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·M Roth, A Rohner
Dec 1, 1994·Scandinavian Journal of Clinical and Laboratory Investigation·M Bläckberg, K Ohlsson
Feb 12, 1998·The American Journal of Physiology·C BloechleJ R Izbicki

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