PMID: 3772370Dec 1, 1986Paper

Studies on a possible functional coupling between presynaptic acetylcholinesterase and high-affinity choline uptake in the rat brain

Journal of Neurochemistry
Maurizio RaiteriA M Caviglia

Abstract

The relationships between presynaptic acetylcholinesterase (AChE) and high-affinity choline uptake (HACU) were investigated using a monolayer of rat cortex synaptosomes in superfusion conditions. The following sets of experiments were performed: determination of [3H]choline ([3H]Ch) uptake during superfusion with [3H]Ch; determination of [3H]Ch uptake during superfusion with acetylcholine (ACh) tritiated in the Ch moiety; evaluation of ACh hydrolysis during superfusion with ACh labelled in the acetate moiety; and comparison of the uptake of [3H]Ch generated by hydrolysis of [3H]ACh with that occurring during superfusion with [3H]Ch. Intact ACh was not taken up by superfused synaptosomes. The uptake of [3H]Ch during superfusion with 1 or 0.1 microM [N-methyl-3H]ACh was two-thirds of that occurring during superfusion with the same concentrations of [3H]Ch. The amount of [3H]Ch produced by hydrolysis during 16 min of superfusion was 1/25 of the amount passing through the synaptosomal monolayer during 16 min of superfusion with [3H]Ch. The results indicate that presynaptic AChE and HACU are located in close proximity to each other on the cholinergic terminal membrane, an observation suggesting the possibility of a functional coupli...Continue Reading

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Citations

Oct 1, 1990·Journal of Neurocytology·M W BrightmanL Chang
Aug 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G RepettoM Repetto
Mar 23, 2004·Neuropharmacology·Luca RaiteriGiambattista Bonanno
Aug 17, 2010·Cellular and Molecular Life Sciences : CMLS·Monica BariMauro Maccarrone
Sep 19, 2002·Drug Development and Industrial Pharmacy·P R Lockman, D D Allen
Oct 1, 1989·Journal of Molecular and Cellular Cardiology·C Nyquist-BattieH Fernandez
Aug 26, 1998·Experimental Neurology·K A DavisM G Blennerhassett
Jul 1, 1989·Circulation Research·C Nyquist-Battie, K Trans-Saltzmann
Dec 12, 2020·Progress in Biophysics and Molecular Biology·Israel Silman

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