PMID: 2487313Dec 1, 1989Paper

Studies on anti-liver cell membrane antibodies in sera of patients with autoimmune chronic active hepatitis

Fukushima Journal of Medical Science
T MoritoR Kasukawa

Abstract

In using the rat hepatocytes isolated and treated with formalin as antigen, IgG class antibodies to the liver cell membrane (LM-Abs) in sera of patients with chronic active hepatitis were examined by both indirect immunofluorescence (IF) and solid-phase enzyme immunoassay (EIA). Specificity of the reaction in IF and EIA was confirmed by an absorption test using different tissues and by inhibition test using the liver specific protein. Indirect IF test using isolated hepatocytes showed the linear staining in eight of 14 sera of patients with autoimmune chronic active hepatitis (CAH). Mean binding values and positive frequency of LM-Abs to the isolated hepatocytes which were detected by EIA were 4.18 +/- 2.99 and 71.4% (10/14) in autoimmune CAH, 3.16 +/- 2.50 and 50% (6/12) in primary biliary cirrhosis, 1.40 +/- 2.12 and 28.6% (4/14) in non-A, non-B, chronic hepatitis (NANB-CH) with serum gamma-globulin showing more than 2.0 gr/dl, 1.02 +/- 1.30 and 0% (0/13) in NANB-CH with gamma-globulin showing less than 2.0 gr/dl, 1.04 +/- 1.20 and 0% (0/15) in HB-CH, and 1.80 +/- 3.60 and 25.0% (3/12) in SLE. Titers of LM-Abs in serum of a autoimmune CAH patient decreased significantly after treatment with prednisolone, but their titers did ...Continue Reading

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