Studies on antibacterial activity of ZnO nanoparticles by ROS induced lipid peroxidation

Colloids and Surfaces. B, Biointerfaces
R K DuttaA V R Reddy

Abstract

Recent studies indicated the role of ROS toward antibacterial activity. In our study we report ROS mediated membrane lipid oxidation of Escherichia coli treated with ZnO nanoparticles (NPs) as supported by detection and spectrophotometric measurement of malondialdehyde (MDA) by TBARS (thiobarbituric acid-reactive species) assay. The antibacterial effects of ZnO NPs were studied by measuring the growth curve of E. coli, which showed concentration dependent bacteriostatic and bacteriocidal effects of ZnO NPs. The antibacterial effects were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Further, antibacterial effect of ZnO NPs was found to decrease by introducing histidine to the culture medium treated with ZnO NPs. The ROS scavenging action of histidine was confirmed by treating histidine to the batch of Escherichia coli+ZnO NPs at the end of the lag phase of the growth curve (Set-I) and during inoculation (Set-II). A moderate bacteriostatic effect (lag in the E. coli growth) was observed in Set-II batch while Set-I showed no bacteriostatic effect. From these evidences we confirmed that the antibacterial effect of bare as well as TG capped ZnO NPs were due to membrane lipid peroxid...Continue Reading

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Citations

Jul 25, 2013·Applied Microbiology and Biotechnology·Rahul S DiggikarBharat B Kale
Aug 6, 2013·Journal of Photochemistry and Photobiology. B, Biology·Raj Kumar DuttaMahesh K Gangishetty
Nov 16, 2012·International Journal of Nanomedicine·Jonathan LelloucheEhud Banin
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May 9, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Marjeta ČepinZorica Crnjak Orel
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Jul 30, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·V Lakshmi Prasanna, Rajagopalan Vijayaraghavan
Jul 22, 2015·Journal of Colloid and Interface Science·Dipika Das, Raj Kumar Dutta
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