PMID: 14392240Jul 1, 1955Paper

Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit

The Journal of Experimental Medicine
A H COONSJ M CONNOLLY

Abstract

A method for the specific histochemical demonstration of antibody in cells and parts of cells is described. It consists of carrying out a two stage immunological reaction on frozen sections of tissues: (a) allowing reaction between antibody in the tissue and dilute antigen applied in vitro, and (b) the detection of those areas where this antigen has been specifically absorbed by means of a precipitin reaction carried out with fluorescein-labelled antibody. Examination under the fluorescence microscope reveals the yellow-green fluorescence of fluorescein over those areas where a precipitate has formed. A study of the hyperimmune rabbit on the first few days after the last of a series of intravenous antigen injections reveals that antibody against human gamma-globulin or ovalbumin is present in groups of plasma cells in the red pulp of the spleen, the medullary areas of lymph nodes, the submucosa of the ileum, and the portal connective tissue of the liver. Because of extensive non-specific reactions, the bone marrow could not be examined. Small amounts of antibody were occasionally visible in cells in the lymphoid follicles of the spleen and lymph nodes, so that a minor contribution by lymphocytes to antibody synthesis cannot be ...Continue Reading

References

Oct 1, 1953·Proceedings of the Society for Experimental Biology and Medicine·W H SHELDON
Jan 1, 1954·Canadian Journal of Biochemistry and Physiology·K F GIRARD, E G MURRAY
Aug 1, 1950·Proceedings of the Society for Experimental Biology and Medicine·E REISSW E EHRICH
Feb 1, 1951·The Journal of Experimental Medicine·A H COONSM H KAPLAN
Mar 1, 1951·Proceedings of the Society for Experimental Biology and Medicine·S P HAYESL P GEBHARDT

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Citations

Feb 10, 1998·The Journal of Comparative Neurology·M VallarinoH Vaudry
Oct 21, 1998·The Journal of Comparative Neurology·S Candiani, M Pestarino
Jul 25, 2000·The Prostate·P A AbrahamssonG Aumüller
Mar 1, 1964·The American Journal of Anatomy·R E MOE
Nov 1, 1967·The American Journal of Anatomy·M R Schwarz
Dec 1, 1965·Arthritis and Rheumatism·J J CondemiJ H Vaughan
Jan 1, 1992·Journal of Clinical Laboratory Analysis·N G CapínL Ortiz-Ortiz
Jan 1, 1987·Cell and Tissue Research·B LöwkvistO Heby
Jan 1, 1969·Histochemie. Histochemistry. Histochimie·M E EtzlerF Moog
Jan 1, 1971·Histochemie. Histochemistry. Histochimie·S Van Noorden, A G Pearse
May 1, 1990·Cell and Tissue Research·M WikgrenP Lindroos
Apr 1, 1990·Cell and Tissue Research·J DominS R Bloom
Dec 1, 1990·Community Outlook·P Keating
Jun 1, 1973·Histochemie. Histochemistry. Histochimie·T Takor Takor, A G Pearse
May 1, 1991·Cell and Tissue Research·S LindskogF Sundler
Jan 1, 1994·Archives of Dermatological Research·R KaufmannC E Klein
Jan 1, 1981·Journal of Cancer Research and Clinical Oncology·M Kraus, G R Krueger
Jan 1, 1983·Archives of Oto-rhino-laryngology·R UddmanF Sundler
Jan 1, 1985·Archives of Oto-rhino-laryngology·R UddmanF Sundler
Jan 1, 1984·Archives of Oto-rhino-laryngology·R UddmanF Sundler

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