Studies on antimalarial drug susceptibility in Colombia, in relation to Pfmdr1 and Pfcrt

Parasitology
E Restrepo-PinedaPedro Cravo

Abstract

In Colombia, Plasmodium resistance to antimalarials such as chloroquine and antifolates is a serious problem. As a result, the national Colombian health authorities are monitoring the efficacy of alternative drugs and schemes. The study of genetic polymorphisms related with drug resistance is required in the region. In vitro responses to chloroquine, quinine, mefloquine, amodiaquine, desethylamodiaquine, artesunate and dihydroartesunate were carried out by HRP ELISA. SNP analysis in Pfcrt and Pfmdr1 genes was performed by PCR-RFLP in 77 samples from the North West region of Colombia. In vitro resistance to chloroquine was high (74%), followed by mefloquine (30%) and desethylamodiaquine (30%). A positive correlation between the IC(50) of paired drugs was also detected. The allele Pfmdr1 N86 (wild) was present in 100% of the samples and 1246Y (mutant) in 92%. However, their presence did not correlate with in vitro drug resistance. Presence of the mutations K76T and N75E in Pfcrt was confirmed in all samples. Analysis of 4 codons (72, 74, 75 and 76) in pfcrt confirmed the presence of the haplotypes CMET in 91% and SMET in 9% of the samples.

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Citations

Nov 13, 2009·The Journal of Infectious Diseases·Fatima NawazPhilip J Rosenthal
Jan 10, 2014·Memórias do Instituto Oswaldo Cruz·Gauri Awasthi, Aparup Das
Sep 29, 2011·Experimental Parasitology·Sarika YadavU N Dwivedi
Feb 9, 2012·Memórias do Instituto Oswaldo Cruz·Gauri AwasthiAparup Das
Sep 9, 2011·Memórias do Instituto Oswaldo Cruz·Juan Pablo QuinteroSócrates Herrera Valencia
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Dec 9, 2020·The American Journal of Tropical Medicine and Hygiene·Lidia Madeline MontenegroTatiana M Lopera-Mesa
Sep 11, 2020·Pharmacology & Therapeutics·Zi-Ning LeiZhe-Sheng Chen

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