PMID: 7011387Dec 4, 1980Paper

Studies on aspartase. VI. Trypsin-mediated activation releasing carboxy-terminal peptides

Biochimica Et Biophysica Acta
N YumotoR Hayashi

Abstract

Aspartase (L-aspartate ammonia-lyase, EC 4.3.1.1) of Escherichia coli, already of full activity, is 3-5-fold activated by a limited proteolysis with trypsin (Mizuta, K. and Tokushige, M. (1976) Biochim. Biophys. Acta 452, 253-261). Structural bases for the activation were investigated. The NH2-termini of the native enzyme and of the trypsin-activated enzyme were found to be equally serine, as analyzed by the dansylation method. However, the COOH-terminal glutamate of the native enzyme was altered to arginine upon activation, as revealed by treatments with carboxypeptidases Y, A and B. The released peptides were obtained by molecular sieve membrane filtration following trypsin activation of the enzyme. The peptides were separated by high voltage paper electrophoresis, and the amino acid composition and the terminal residues were determined. The results showed that one or a few related peptides consisting of 7-17 residues were released from the COOH-terminal upon activation. The circular dichroism spectrum of the enzyme suggested that the helical content of the activated enzyme was about 5% less than that of the native enzyme, an indication that the trypsin-activated enzyme has a somewhat looser conformation than the native enzym...Continue Reading

References

Nov 17, 1975·Biochemical and Biophysical Research Communications·K Mizuta, M Tokushige
Nov 8, 1976·Biochimica Et Biophysica Acta·K Mizuta, M Tokushige
Sep 22, 1975·Biochimica Et Biophysica Acta·K Mizuta, M Tokushige
Jan 1, 1977·Methods in Enzymology·R Hayashi
Mar 1, 1972·Archives of Biochemistry and Biophysics·S TranielloR L Horecker
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS
Dec 14, 2005·Archives of Physical Medicine and Rehabilitation·Jaana M SarajuuriJuhani S Vilkki
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G R FlemingG W Robinson
Feb 27, 2009·Journal of Virology·Christopher B WhitehurstJoseph S Pagano
Jan 1, 1972·Methods in Enzymology·W R Gray

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Citations

Mar 25, 1985·Nucleic Acids Research·J S TakagiY Shimura
Feb 11, 1982·Biochemical and Biophysical Research Communications·N YumotoM Tokushige
Sep 23, 1997·Biochemical and Biophysical Research Communications·M M JayasekeraR E Viola
May 31, 1991·Biochemical and Biophysical Research Communications·S MuraseM Tokushige
Jan 1, 1987·Annals of the New York Academy of Sciences·Y H ChengS Q Wang
Nov 28, 1983·Biochimica Et Biophysica Acta·N Yumoto, M Tokushige
Oct 13, 1981·Biochimica Et Biophysica Acta·Y WatanabeG Eguchi

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