PMID: 6969692Jan 1, 1980Paper

Studies on beta-lactamase in Bacteroides fragilis

Infection
C E NordB Olsson

Abstract

Fifteen strains of Bacteroides fragilis, five highly resistant, five moderately resistant and five sensitive to benzylpenicillin and cephaloridine, were tested for beta-lactamase production. In the highly resistant and the moderately resistant groups of strains, a correlation between formation of beta-lactamase and MIC-values was found. Culture under controlled conditions in stirred fermentors gave higher yields of beta-lactamase than static cultures in bottles. The enzyme had its maximum activity at pH 5.0 and was stable between pH 5.5-8.5. Isoelectric focusing in polyacrylamide gels gave an isoelectric point of 4.9 +/- 0.2 for the enzyme. In the presence of the beta-lactamase inhibitors clavulanic acid and CP-45.899 at concentrations of 1 microgram/ml, the susceptibility to cephaloridine increased fourfold or more in the beta-lactamase-producing strains. Crypticity measurements (beta-lactamase activity broken/intact cells) with cephaloridine as substrate could indicate a diffusion barrier in the cell wall.

References

May 1, 1976·Antimicrobial Agents and Chemotherapy·B OlssonT Wadström
Mar 1, 1978·Antimicrobial Agents and Chemotherapy·M L Britz, R G Wilkinson
Oct 7, 1977·Medical Microbiology and Immunology·B OlssonC E Nord
Mar 1, 1977·The Journal of Infectious Diseases·S M Finegold
Jul 1, 1978·The Journal of Antimicrobial Chemotherapy·M H Richmond
Jun 1, 1976·The Journal of Antimicrobial Chemotherapy·R B Sykes, M Matthew
Apr 1, 1972·Antimicrobial Agents and Chemotherapy·C H O'CallaghanA H Shingler

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Citations

Sep 26, 1997·Antimicrobial Agents and Chemotherapy·M E FalagasD R Snydman

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