PMID: 9425104Feb 28, 1998Paper

Studies on glutathione S-transferases important for sperm function: evidence of catalytic activity-independent functions

The Biochemical Journal
B GopalakrishnanC Shaha

Abstract

Our earlier studies reported the identification of a rat testicular protein of 24 kDa with significant similarity at the N-terminus with Mu class glutathione S-transferases (GSTs). Treatment of goat sperm with antisera against this protein identified immunoreactive sites on the spermatozoa and inhibited in vitro fertilization of goat oocytes by the antibody-treated sperm. The above observations indicated the presence of GST-like molecule(s) important for fertility related events on goat spermatozoa. In this study, we report the purification of goat sperm GSTs (GSP1) which were purified by glutathione affinity chromatography and were enzymically active towards 1-chloro-2,4,-dinitrobenzene, a general GST substrate, and ethacrynic acid, a substrate for Pi class GSTs. GSP1 resolved into three major components on reverse-phase HPLC: peaks 1 and 2 with molecular masses of 26.5 kDa and peak 3 with a molecular mass of 25.5 kDa, as determined by SDS/PAGE. Multiple attempts to obtain N-terminal sequences of the first two peaks failed, indicating N-terminal block; however, they reacted to specific anti-Mu-GST antisera on Western blots and ELISA, and not to anti-Pi-GST antisera, which provides evidence for the presence of Mu-GST-reactive s...Continue Reading

Citations

Apr 26, 2006·American Journal of Reproductive Immunology : AJRI·Agnieszka ParadowskaWalter Krause
Jun 13, 2008·Journal de la Société de biologie·Catherine SerresPierre Jouannet
Jan 29, 2013·Human Reproduction·F M PetitJ Auer
Nov 18, 2014·Biochemical Society Transactions·François M PetitJana Auer
Dec 7, 2019·Cellular and Molecular Life Sciences : CMLS·Marc LlavaneraMarc Yeste
Nov 9, 2017·Science Progress·Constance J Jeffery
Aug 9, 2019·Journal of Animal Science and Biotechnology·Marc LlavaneraMarc Yeste
Mar 8, 2003·Archives of Andrology·E E MontielE A Castellon
Dec 6, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Constance J Jeffery
Dec 3, 2020·Drug Metabolism Reviews·Philip G Board, M W Anders
Mar 19, 2021·Frontiers in Cell and Developmental Biology·Marc LlavaneraMarc Yeste
Nov 21, 2000·Free Radical Biology & Medicine·A V Rao, C Shaha
Aug 3, 2021·Frontiers in Cell and Developmental Biology·Mari-Carmen Fuentes-AlberoFrancisco Alberto García-Vázquez

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