Studies on human platelet glycolysis. Effect of glucose, cyanide, insulin, citrate, and agglutination and contraction on platelet glycolysis

The Journal of Clinical Investigation
S Karpatkin

Abstract

Evidence for a metabolically active plasma-free platelet system is presented. Glycogenolysis was found to be a potent pathway for lactate production. Aerobic glycolysis constituted a major fraction of glucose metabolized. Both insulin and cyanide increased lactate production in the presence of glucose. The Krebs cycle appeared to be operative for ATP synthesis when citrate was used as substrate. The first stages of gluconeogenesis were noted to be present. Glucose uptake contributed to increased lactate production.Thrombin, epinephrine, and ADP resulted in platelet agglutination and contraction and increased platelet glycogenolysis (phosphorylase activity). Epinephrine appeared to be a more potent activator of phosphorylase, resulting in a 70% increase in glucose 6-phosphate levels. Thrombin and epinephrine both increased glucose uptake and lactate production. Glucose uptake decreased in the presence of ADP. Except for incubations with epinephrine, glucose 6-phosphate remained constant under conditions in which lactate flux increased 3-fold and glucose uptake increased 2-fold. Thrombin, epinephrine, and ADP decreased ATP levels in the presence or absence of glucose.

References

Feb 18, 1964·Biochemical and Biophysical Research Communications·I A RoseE L O'Connell
Jul 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·W H DANFORTH CORICF
Jan 1, 1964·British Journal of Haematology·J R MITCHELL, A A SHARP
May 1, 1964·The Journal of Clinical Investigation·R H ASTER, J H JANDL

❮ Previous
Next ❯

Citations

May 1, 1986·Diabetologia·D B JonesR J Prescott
Dec 8, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·L G Svirklys, W J O'Sullivan
Jan 1, 1986·The International Journal of Biochemistry·A J VerhoevenJ W Akkerman
Mar 1, 1982·Journal of the Neurological Sciences·R M Mangano, R Schwarcz
Nov 1, 1985·Molecular and Cellular Endocrinology·H Setiadi, A C Herington
Jul 28, 1973·Biochimica Et Biophysica Acta·P D Zieve, M Schmukler
Jun 12, 1969·The New England Journal of Medicine·A J Marcus
Jun 1, 1968·British Journal of Haematology·P B LoderG C De Gruchy
Aug 1, 1970·British Journal of Haematology·S Karpatkin, A Charmatz
Oct 27, 1972·Annals of the New York Academy of Sciences·M Steiner
Oct 27, 1972·Annals of the New York Academy of Sciences·S Karpatkin
Jan 1, 1970·The Journal of Clinical Investigation·P Cohen, B Wittels
Feb 1, 1973·The Journal of Clinical Investigation·D Deykin
May 1, 1979·The Journal of Clinical Investigation·A S HajekW H Daughaday
Jun 1, 1979·The Journal of Clinical Investigation·M A ShumanJ W Fenton
Apr 1, 1981·The Journal of Clinical Investigation·G C YehJ M Phang
Jun 6, 2015·Thrombosis Research·Michael Isaäc MeestersChrista Boer
Apr 16, 2011·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Maria I C Gyongyossy-Issa
May 11, 1970·Biochemical and Biophysical Research Communications·A DeisserothN R Shulman
Dec 1, 1972·Archives of Biochemistry and Biophysics·L Salganicoff, M H Fukami
Jan 1, 1973·Metabolism: Clinical and Experimental·N NakamuraP Walter
Sep 10, 1968·Saishin igaku. Modern medicine·K Fukutake, M Ukita
Feb 1, 1981·British Journal of Haematology·K K WuY C Chen
Sep 1, 1970·Biochemical Pharmacology·H M Solomon, Z N Gaut
Feb 27, 2014·The International Journal of Biochemistry & Cell Biology·Luiz F Garcia-Souza, Marcus F Oliveira
Apr 1, 1971·British Journal of Haematology·H BaadenhuijsenC Haanen
Oct 1, 1968·Archives of Biochemistry and Biophysics·C M Grossman, F Bartos
Aug 5, 2017·Thrombosis and Haemostasis·Trevor P FidlerE Dale Abel
Jul 1, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Trevor P FidlerE Dale Abel
Aug 29, 2018·Journal of Thrombosis and Haemostasis : JTH·M AibibulaR G Sturmey
Aug 1, 1976·Biulleten' eksperimental'noĭ biologii i meditsiny·I L LisovskaiaR A Markosian

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.