Studies on human porin: XIII. The type-1 VDAC 'porin 31HL' biotinylated at the plasmalemma of trypan blue excluding human B lymphocytes

FEBS Letters
C JakobN Hilschmann

Abstract

In 1989, we demonstrated for the first time the expression of the VDAC 'Porin 31HL' in the plasmalemma of human B lymphocytes, then giving first evidence of a multi-topological expression of VDAC. Meanwhile, data from this and other laboratories support our proposal of a multi-compartment distribution of the channel in mammalian tissues. Here, by biotinylation of plasmalemma-integrated proteins of proven living B lymphocytes, followed by two-dimensional electrophoresis, immuno- and streptavidin affinity blotting, we show that part of the channel molecules can be labelled at the outer membrane of the cells. Thus, by a relevant approach our results invalidate objections concerning putative cross-reactivity of anti-human Type-1 porin antibodies with non-VDAC proteins at the outer cell membrane.

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Citations

Apr 12, 2008·Purinergic Signalling·Ravshan Z Sabirov, Yasunobu Okada
Apr 3, 2009·The Journal of Physiological Sciences : JPS·Ravshan Z Sabirov, Yasunobu Okada
Feb 25, 2000·The International Journal of Biochemistry & Cell Biology·P SteinackerN Hilschmann
Oct 13, 2004·The Journal of General Physiology·Seiko F OkadaRichard C Boucher
Mar 30, 2010·Molecular Aspects of Medicine·Varda Shoshan-BarmatzNir Arbel
Oct 12, 2011·Biochimica Et Biophysica Acta·Ravshan Z Sabirov, Petr G Merzlyak
Jul 5, 2015·Veterinary Parasitology·Elba Rodríguez-HernándezMinerva Camacho-Nuez
Sep 4, 2004·Biophysical Journal·Ravshan Z Sabirov, Yasunobu Okada
Feb 27, 2010·FEBS Letters·Vito De PintoAlfons Lawen
Apr 28, 2016·Biochimica Et Biophysica Acta·Shamim Naghdi, György Hajnóczky
Nov 16, 2018·British Journal of Pharmacology·Luigi LeanzaIldiko Szabo
Nov 18, 2005·The Journal of Biological Chemistry·Ravshan Z SabirovWilliam J Craigen
Apr 3, 2014·Physiological Reviews·Ildiko Szabo, Mario Zoratti
Oct 24, 2003·The Journal of Biological Chemistry·Mark A BakerAlfons Lawen

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