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Studies on immobilized trypsin in high concentrations of organic solvents

Biotechnology and Bioengineering

Jan 1, 1976

Howard H Weetall, W P Vann

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Abstract

Trypsin was covalently immobilized on porous glass in the presence and absence of a specific substrate and reacted in various organic solvents of different dielectric constants. Optimum solvent concentration, pH profile, Km(app), Vmax(app), productivity versus temperature, activity, and...read more

Mentioned in this Paper

Arginine hydrochloride
Fluorescence Spectroscopy
Organic solvent product
Acids
Hydrolysis
Carboxyl Group
Trypsin
PH Profile Measurement
Blood Enzyme Activity (Lab Test)
Hydrogen-Ion Concentration
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  • Studies on immobilized trypsin in high concentrations of organic solvents

    Biotechnology and Bioengineering

    Jan 1, 1976

    Howard H Weetall, W P Vann

    PMID: 2353

    DOI: 10.1002/bit.260180109

    Abstract

    Trypsin was covalently immobilized on porous glass in the presence and absence of a specific substrate and reacted in various organic solvents of different dielectric constants. Optimum solvent concentration, pH profile, Km(app), Vmax(app), productivity versus temperature, activity, and...read more

    Mentioned in this Paper

    Arginine hydrochloride
    Fluorescence Spectroscopy
    Organic solvent product
    Acids
    Hydrolysis

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