Studies on interaction of norbixin with DNA: multispectroscopic and in silico analysis

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Amrita AnantharamanRamamoorthy Siva

Abstract

The interaction of food colorant norbixin with calf thymus DNA (CTDNA) was investigated through UV-Visible spectroscopy, Fourier Transform Infrared (FTIR), Circular Dichroism (CD), Nuclear Magnetic Resonance (NMR), DNA melting studies, electrophoretic analysis, histological staining technique and molecular docking studies. The results indicated that norbixin interacted with CTDNA by partial intercalation mode. The binding constant (K) of norbixin with CTDNA was calculated to be 5.08×10(5) Mol(-1) L. FTIR and CD studies were coupled with (1)H NMR spectra revealed that norbixin intercalates partially and binds to the groove's, phosphate group, deoxyribose sugar of DNA and also induces conformational transition of B-form to A-form DNA. Agarose gel electrophoretic and histological staining technique results further prove that, norbixin specifically binds to the DNA in the cell. Moreover, molecular docking studies on the specific binding of norbixin with CTDNA have exhibited lowest conformation energy score of -3.2. Therefore, this food colorant has the ability to interact with DNA and it could emerge as a promising class of natural DNA targeted therapeutic.

References

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Aug 15, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·H NoppeH F De Brabander
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Feb 11, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Swarup RoyTapan Kumar Das

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Citations

Jul 19, 2015·Journal of Bioscience and Bioengineering·Amrita AnantharamanRamamoorthy Siva
Feb 26, 2016·Nucleosides, Nucleotides & Nucleic Acids·Hridya HemachandranRamamoorthy Siva
May 9, 2015·Archives of Biochemistry and Biophysics·Sayeed Ur RehmanMohammad Tabish
May 27, 2017·Nucleosides, Nucleotides & Nucleic Acids·Neda Hosseinpour MoghadamReza Golbedaghi
Nov 20, 2018·Luminescence : the Journal of Biological and Chemical Luminescence·Kishor B Kale, Divya P Ottoor
Feb 15, 2019·Nature·Christopher P StanleyRoland Stocker
Jul 3, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Hui GuoXun Tuo
Dec 6, 2016·Journal of Molecular Recognition : JMR·Razique AnwerTasneem Fatma
May 28, 2021·3 Biotech·Subramanian PrathibaGurunathan Jayaraman
Jan 10, 2018·Journal of Medicinal Chemistry·Bose Muthu RamalingamArasambattu K Mohanakrishnan
Sep 10, 2021·Luminescence : the Journal of Biological and Chemical Luminescence·Guofang ZengYing Guo

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