PMID: 1201690Dec 10, 1975Paper

Studies on Macronuclear DNA from Paramecium aurelia

Chromosoma
D J Cummings

Abstract

Macronuclear DNA was isolated from purified macronuclei of Paramecium aurelia and the size distribution was determined with regard to growth phase and method of extraction. DNA molecules as long as 105 microns and as short as 0.2 microns were observed. It was concluded that the method of extraction affected the observed length of DNA extracted and that macronuclear DNA isolated from cells in balanced growth was less susceptible to nuclease degradation than was DNA isolated from cells in stationary phase. Renaturation studies were performed on macronuclear DNA and a kinetic complexity of 22-times E. coli DNA was determined. This value was similar to those values reported for Tetrahymena and Stylonychia macronuclear DNA. Correcting for GC base content yielded a kinetic complexity for Paramecium macronuclear DNA of 11-times E. coli DNA which corresponded to 3 X 10(10) daltons. There would be about 1400 copies of a unit genome of this complexity within each newly replicated macronucleus. Density gradient analysis indicated that the genes coding for ribosomal RNA had a greater density in CsCl than the bulk DNA. Molecular hybridization studies indicated that the genes coding for 25 S RNA represented 0.14 percent of the total macronuc...Continue Reading

References

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Citations

Aug 1, 1996·Journal of Cellular Biochemistry·C M Clemson, J B Lawrence
Jan 1, 1981·Chromosoma·G SteinbrückD Ammermann
Jan 1, 1981·Chromosoma·C SamuelJ Sommerville
Jan 1, 1980·Chromosoma·C McTavish, J Sommerville
Feb 25, 1983·Journal of Immunological Methods·P V Subba RaoD D Metcalfe
Sep 1, 1976·Mechanisms of Ageing and Development·V Sundararaman, D J Cummings
Nov 1, 1984·Mechanisms of Ageing and Development·K J Aufderheide
Nov 1, 1985·Mechanisms of Ageing and Development·K J Aufderheide, M V Schneller
Aug 30, 1982·Biochimica Et Biophysica Acta·J Sommerville, C McTavish
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R C Findly, J G Gall
Mar 1, 1980·The Journal of Cell Biology·R C Findly, J G Gall
Dec 1, 1978·Nucleic Acids Research·H J LippsG Steinbrück
Jan 29, 2000·The Journal of Eukaryotic Microbiology·A M Keller, J Cohen
Jul 15, 1977·Biochimica Et Biophysica Acta·D E HrubyD J Cummings
Jan 26, 2016·European Journal of Protistology·Zoya I Uspenskaya, Alexander L Yudin
Jun 1, 1994·Microbiological Reviews·D M Prescott

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