Studies on molecular interactions between nalidixic acid and liposomes

International Journal of Pharmaceutics
Marianna BudaiPál Gróf

Abstract

The interaction between nalidixic acid sodium salt (NANa) and liposomes prepared from alpha-L-dipalmitoyl-phosphatidylcholine (DPPC) or from its binary mixture with dioleoyl-phosphatidylcholine (DOPC) was studied with differential scanning calorimetry (DSC) and electron paramagnetic resonance (EPR) spectroscopy. We evaluated the role of broadband ultraviolet-B (UV-B) irradiation on the molecular interactions between the lipids and the NANa, and determined the decay-kinetics of the incorporated spin labeled fatty-acid free radicals. Multilamellar and unilamellar vesicles were prepared by sonication and extrusion. The entrapment efficiencies were determined spectrophotometrically. The size-distribution of the liposomes and its change in time was checked by dynamic light scattering (DLS). Our results indicate that NANa mainly interacts with lipid head groups. However, its effect and presumably the formation of the free radicals, induced by broadband ultraviolet-B, is not localized only to the head group region of the lipid molecules. Depending on DOPC content, interaction between the NANa and the lipids modifies the phase-transition parameters of the liposome dispersions.

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Citations

May 9, 2014·Journal of Basic and Clinical Pharmacy·Chamari WijesooriyaIlona Petrikovics
Jan 4, 2007·Analytical and Bioanalytical Chemistry·Patrícia NevesPaula Gameiro
Aug 28, 2010·Nanotoxicology·Ilona PetrikovicsMaria Szilasi
Apr 30, 2011·International Journal of Pharmaceutics·Nina Dragicevic-CuricAlfred Fahr
Aug 23, 2008·International Journal of Pharmaceutics·C CaddeoJ Kristl
Oct 20, 2015·Journal of Colloid and Interface Science·Ernest SooAmirali Popat
Nov 18, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Szabolcs BéniPál Gróf
May 14, 2011·Pharmaceutical Development and Technology·Patrícia SeverinoMaria H A Santana
Nov 22, 2008·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Kristóf KovácsKrisztina Ludányi
Apr 1, 2005·Journal of Microencapsulation
Dec 14, 2018·Toxicology Research·Xiaoqi LiLisha You
Sep 15, 2017·AAPS PharmSciTech·Karthik Yadav JangaS Narasimha Murthy

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