PMID: 7523940Nov 1, 1994Paper

Studies on mutations in male germ cells of transgenic mice following exposure to isopropyl methanesulfonate, ethylnitrosourea or X-ray

Mutation Research
M KatohT Shibuya

Abstract

Transgenic mice have recently been used for mutagenesis assays in vivo. The present study was undertaken to clarify whether such assays can detect mutations induced after treatment of male germ cells in mouse with isopropyl methanesulfonate (iPMS), ethylnitrosourea (ENU) or X-ray irradiation. The transgenic mice used for assay are Muta Mouse (MM) strain, which carries 80 copies of the bacterial lacZ gene per cell as targets for mutagenesis. Male MM animals were given a single intraperitoneal injection of 200 mg/kg iPMS, 150 mg/kg ENU or were irradiated with 500 rads of X-rays. Vasa deferential sperm, caudal epididymal sperm and/or whole testes were extracted at various times after treatment with each agent. After the genomic DNA was extracted from each tissue, mutation analysis at the lacZ locus was carried out by the method of Myhr et al. The spontaneous lacZ- mutant frequencies were on the order of 10(-5)-10(-6). The lacZ- mutant frequencies in all treatment groups were increased over the control animals. The iPMS-induced mutant frequency in postmeiotic stages was low. However, ENU induced relatively high mutant frequencies in the spermatogonia. X-rays induced mutant frequencies in the late spermatid and early spermatid stage...Continue Reading

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W M GenerosoS W Huff
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W L RussellE L Phipps
Feb 1, 1979·Mutation Research·U H Ehling, A Neuhäuser
Jan 1, 1991·Environmental and Molecular Mutagenesis·B C Myhr
Jan 1, 1991·Environmental and Molecular Mutagenesis·S W KohlerJ M Short
Dec 1, 1990·Genetic Analysis, Techniques and Applications·S W KohlerJ M Short
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J A GossenJ Vijg
Apr 27, 1989·Nature·W R Brown
Jan 1, 1987·Environmental and Molecular Mutagenesis·G A Sega, J G Owens
Sep 1, 1973·Toxicology·A Léonard, N Gilliavod
Jan 1, 1994·Environmental and Molecular Mutagenesis·W W PiegorschM D Shelby
Apr 1, 1960·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·E F OAKBERG, R L DIMINNO

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Citations

Oct 1, 1995·Mutation Research·X B ZhangJ A Heddle
Oct 1, 1995·Mutation Research·H J MartusJ Vijg
Dec 13, 2000·Mutation Research·T NohmiK Masumura
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·C A WalterR B Walter
Oct 18, 2000·Proceedings of the National Academy of Sciences of the United States of America·R N WinnS L Muller
Dec 19, 2002·Environmental and Molecular Mutagenesis·Lois B BarnettSusan E Lewis
Aug 6, 2005·Mutation Research·Iain B LambertGeorge R Douglas
Oct 2, 2002·Environmental and Molecular Mutagenesis·Fumio YatagaiNobuhisa Fukunishi
Jan 1, 1996·Environmental and Molecular Mutagenesis·T NohmiT Sofuni
Jan 1, 1996·Environmental and Molecular Mutagenesis·J A HeddleY Paashuis-Lew

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