Studies on native ribosomal subunits from rat liver. Evidence for activities associated with native 40S subunits that affect the interaction with acetylphenylalanyl-tRNA, methionyl-tRNAf, and 60S subunits.

Biochemistry
I SadnikK Moldave

Abstract

The binding of the initiator tRNA Met-tRNAf, and of acetylphenylalanyl-tRNA, has been examined with rat liver 40S subunits derived from 80S ribosomes by dissociation with native 40S subunits sedimented from the postmicrosomal fraction and with native 40S subunits extracted with high salt-containing solutions. Binding of Met-tRNAf and acetylphenylalanyl-tRNA to derived and to salt-extracted native 40S subunits is observed in the presence of the appropriate polynucleotide template and a highly purified binding factor obtain from the soluble fraction of rat liver homogenates (R.L. IF-1). Native 40S subunits bind acetylphenylalanyl-tRNA in a reaction that requires poly(U) but not exogenous binding factor; however, Met-tRNAf is not bound to native subunits, even when supplemented with the soluble binding factor, or under conditions where factor-independent, high Mg2+-stimulated binding is observed with the derived and the salt-washed native 40S subunits. The extract obtained from native 40S subunits promotes the binding of acetylphenylalanyl-tRNA but not Met-tRNAf to derived and to salt-extracted native subunits. The addition of native 40S extract to incubations containing R.L. IF-1, Met-tRNAf, and derived 40S subunits, inhibits the...Continue Reading

Citations

Jun 8, 2001·Biochemical and Biophysical Research Communications·H ZhongJ W Simons
Sep 11, 2001·Biochemical and Biophysical Research Communications·A SodhiJ S Gutkind
Mar 30, 1999·Journal of Pharmaceutical and Biomedical Analysis·V SewramD A Mulholland
Mar 31, 1999·Trends in Biochemical Sciences·C V Dang, G L Semenza
Feb 1, 1978·European Journal of Biochemistry·M Fresno, D Vázquez
Jun 1, 1979·Journal of Neurochemistry·P T Van der Saag, S Bordin
Mar 31, 1981·Biochemical and Biophysical Research Communications·J S Hutchison, K Moldave
Oct 1, 1982·Journal of Cellular Physiology·R LotanK Moldave
Jan 1, 1978·CRC Critical Reviews in Biochemistry·B Safer, W F Anderson

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