Studies on neuromuscular blockade by boldine in the mouse phrenic nerve-diaphragm

Japanese Journal of Pharmacology
J J KangW M Fu

Abstract

The effects of boldine [(S)-2,9-dihydroxyl-1,10-dimethoxy-aporphine], a major alkaloid in the leaves and bark of Boldo (Peumus boldus Mol.), on neuromuscular transmission were studied using a muscle phrenic-nerve diaphragm preparation. Boldine at concentrations lower than 200 microM preferentially inhibited, after an initial period of twitch augmentation, the nerve-evoked twitches of the mouse diaphragm and left the muscle-evoked twitches unaffected. The twitch inhibition could be restored by neostigmine or washout with Krebs solution. The twitches evoked indirectly and directly were both augmented initially, suggesting that the twitch augmentation induced by boldine was myogenic. Boldine inhibited the acetylcholine-induced contraction of denervated diaphragm dose-dependently with an IC50 value of 13.5 microM. At 50 microM, boldine specifically inhibited the amplitude of the miniature end plate potential. In addition, boldine was similar to d-tubocurarine in its action to reverse the neuromuscular blocking action of alpha-bungarotoxin. These results showed that the neuromuscular blockade by boldine on isolated mouse phrenic-nerve diaphragm might be due to its direct interaction with the postsynaptic nicotinic acetylcholine rece...Continue Reading

References

Jan 1, 1992·Annual Review of Pharmacology and Toxicology·M Bertolino, R R Llinás
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·O D UchitelR Llinás
Dec 1, 1991·Planta medica·H SpeiskyL J Núñez-Vergara
Oct 1, 1988·Trends in Neurosciences·R W TsienA P Fox
Jan 1, 1989·Annals of the New York Academy of Sciences·D YoshikamiB M Olivera
Mar 29, 1988·European Journal of Pharmacology·D J DooleyH Osswald
May 1, 1993·The Journal of Pharmacy and Pharmacology·M D IvorraP D'Ocon
Jan 1, 1994·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·H Speisky, B K Cassels
Feb 9, 1993·European Journal of Pharmacology·M D IvorraM P D'Ocon
Dec 1, 1996·British Journal of Pharmacology·S ChuliáC Advenier
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE
Nov 28, 1951·The Journal of Physiology·P FATT, B KATZ
Mar 1, 1946·British Journal of Pharmacology and Chemotherapy·E BULBRING

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Citations

Oct 14, 2005·Chemico-biological Interactions·Peter O'BrienHernán Speisky

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