Studies on neurosteroids XVIII LC-MS analysis of changes in rat brain and serum testosterone levels induced by immobilization stress and ethanol administration

Steroids
Tatsuya HigashiKazutake Shimada

Abstract

The liquid chromatography-mass spectrometry (LC-MS) methods were developed and validated for the determination of testosterone (T) in the brain and serum of rats and of 5alpha-androstane-3alpha,17beta-diol (ADIOL), a metabolite of T, in the brain of rats. After derivatization of T with 2-hydrazino-1-methylpyridine and of ADIOL with p-nitrobenzoyl chloride, the detection sensitivities of T and ADIOL using LC-MS were increased 70- and 400-times superior to those of intact T and intact ADIOL, respectively. Those LC-MS methods are specific and reliable for the analysis of trace amounts of T and ADIOL in small amounts of samples. The animal studies using the developed methods showed that the brain and serum levels of T and the brain levels of ADIOL were not changed by stress or ethanol administration but the concentration ratio of the brain T to serum T in the stressed rats was higher than that in untreated rats. The low levels of endogenous AIDOL in brain of stressed and unrestrained rats found in this study demonstrated that the contribution to anesthetic and anxiolytic effects of ADIOL via gamma-aminobutyric acid type A receptors may be negligible.

References

May 25, 1991·Proceedings of the National Academy of Sciences of the United States of America·R H PurdyS M Paul
Dec 7, 2002·Cognitive, Affective & Behavioral Neuroscience·C A Frye, A M Seliga
Nov 5, 2003·Analytical and Bioanalytical Chemistry·Tatsuya Higashi, Kazutake Shimada
Feb 8, 2005·Progress in Neuro-psychopharmacology & Biological Psychiatry·Bernardo O Dubrovsky
May 28, 2005·Psychoneuroendocrinology·Alonso Fernández-Guasti, Lucía Martínez-Mota
Oct 11, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tatsuya HigashiKazutake Shimada

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Citations

Aug 11, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Tatsuya HigashiKazutake Shimada
Sep 11, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Seung-Jin KimYong-Ill Lee
Nov 28, 2006·Biochimie·Yuqin WangWilliam J Griffiths
Jan 31, 2007·Journal of Mass Spectrometry : JMS
Apr 1, 2020·Biology of Sex Differences·Phong DuongRebecca L Cunningham
May 10, 2018·Frontiers in Endocrinology·Yasushi Hojo, Suguru Kawato

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