PMID: 13575683Oct 1, 1958Paper

Studies on persistent infections of tissue cultures. I. General aspects of the system

The Journal of Experimental Medicine
G HENLEW HENLE

Abstract

Inoculation of the MCN and Lung-To lines of human cells in continuous culture with Newcastle disease (NDV), mumps, or 6-6 viruses led to slight cytopathic effects (CPE) if the multiplicity of infection exceeded one. On second passage or with smaller initial inocula no CPE became apparent. The viruses multiplied, however, as determined by titrations in HeLa cultures or chick embryos. Indeed, persistently infected sublines of MCN and Lung-To were readily established without resort to special manipulations and some of these have been carried now for over 18 months on the same media and schedules as the uninfected parent strains. The viruses were found to be associated mainly with the cells and only 1, or at most 10 per cent of it was detectable in the media. The titers obtained were always low in relation to the available cell population. Reduction or even omission of the horse serum component in the media or ultraviolet irradiation of the cultures did not increase the yield of virus, and CPE became apparent only when similarly treated, uninfected cultures were, likewise, affected by the manipulations. The persistently infected cultures differed from their uninfected counterparts in that they exhibited (a) decreased cellular growt...Continue Reading

References

Nov 1, 1954·Proceedings of the Society for Experimental Biology and Medicine·G HENLEA GIRARDI
Nov 1, 1955·The Journal of Experimental Medicine·W W ACKERMANN, H KURTZ
Dec 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·A S KAPLAN, J L MELNICK
Jan 1, 1956·The Journal of Infectious Diseases·S HOTTA, C A EVANS
Mar 1, 1956·Proceedings of the Society for Experimental Biology and Medicine·H EAGLER J HUEBNER
Jul 1, 1956·Proceedings of the Society for Experimental Biology and Medicine·A D HEGGIE, H R MORGAN
Jul 1, 1956·Proceedings of the Society for Experimental Biology and Medicine·D L LAGERBORG, H E PEARSON
Apr 19, 1957·Annals of the New York Academy of Sciences·W W ACKERMANN
Jan 30, 1958·The New England Journal of Medicine·R M CHANOCKR J HUEBNER
Jun 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·A F GRAHAM, L SIMINOVITCH

❮ Previous
Next ❯

Citations

Jan 1, 1967·Archiv für die gesamte Virusforschung·E Norrby
Jan 1, 1961·Archiv für die gesamte Virusforschung·A R BEASLEYM M SIGEL
Jan 1, 1961·Archiv für die gesamte Virusforschung·K CANTELL
Jan 1, 1968·Archiv für die gesamte Virusforschung·E Katz, N Goldblum
Jan 1, 1963·Archiv für die gesamte Virusforschung·I GRESSER
Jan 1, 1965·Archiv für die gesamte Virusforschung·T MAGUIRE, J A MILES
Jan 1, 1971·Archiv für die gesamte Virusforschung·G C Mohanty, G E Cottral
Jan 1, 1978·Archives of Virology·J M MezencioR R Golgher
Jan 1, 1978·Archives of Virology·J S Wolinsky, W G Stroop
Jan 1, 1979·Archives of Virology·R M Friedman, J M Ramseur
Jun 1, 1976·Medical Microbiology and Immunology·R K Rima, S J Martin
Jan 23, 1960·Nature·C GIL FERNANDEZ
Jun 21, 1962·The New England Journal of Medicine·M HO
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J E Blalock, G E Gifford
Oct 1, 1959·The Journal of Experimental Medicine·W H MURPHY, R ARMSTRONG
Jul 23, 1960·British Medical Journal·D S NELSON, P M DE BURGH
Mar 23, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Francesco GiansantiLucilla Seganti
Apr 28, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Francesco GiansantiGiovanni Antonini
Apr 1, 1962·The Journal of Experimental Medicine·A M LERNERM FINLAND
Oct 30, 1981·Virology·M McCarthyR A Lazzarini
Apr 23, 2005·Biochemical and Biophysical Research Communications·Francesco GiansantiGiovanni Antonini
Feb 1, 1961·Virology·A ISAACSG HITCHOCK
Jan 7, 2014·Journal of Virology·Christophe RamièreOlivier Diaz
Dec 1, 1983·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·M Degré, L A Glasgow
Jun 1, 1967·Bacteriological Reviews·K Paucker, M Boxaca

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.