PMID: 9165668May 1, 1997Paper

Studies on polymorphonuclear leukocyte bactericidal function: II. The role of oxidative stress

Shock
H H Simms, R D'Amico

Abstract

We have previously detailed the effects of exogenous cytokines (IL-1 beta, TNF-alpha, and IL-8) on polymorphonuclear leukocyte (PMN) bactericidal function during normoxia. In these studies, we have investigated the effects of hypoxia +/- reoxygenation independently and with IL-1 beta, TNF-alpha, and IL-8 on PMN bactericidal activity. Hypoxia in and of itself did not significantly alter PMN bactericidal activity against Escherichia coli or Staphylcoccus aureus; however, after 2 h of reoxygenation (H/R), PMN bactericidal activity against E. coli was significantly reduced compared with levels seen after 2 h of hypoxia. Similar to what was observed during normoxia, TNF-alpha, IL-1 beta, and IL-8 increased PMN bactericidal activity during hypoxia compared with buffer control PMN for S. aureus but not E. coli after 4 h of hypoxia. Following H/R, neither TNF-alpha, IL-1 beta, nor IL-8 reversed the decline in bactericidal activity induced by reoxygenation alone. Monoclonal antibodies that blocked the functional epitope of particular cytokine receptors demonstrated that during both hypoxia and H/R IL-1 beta R type I, IL-8R type A, and TNF-alpha R p60 were the predominant receptors responsible for mediating the bactericidal effect of the...Continue Reading

Citations

Mar 13, 2001·The Journal of Maternal-fetal Medicine·K M Hanlon-Lundberg, R S Kirby

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