PMID: 41578Dec 4, 1979

Studies on purine transport and on purine content in vacuoles isolated from Saccharomyces cerevisiae

Biochimica Et Biophysica Acta
M Nagy

Abstract

The transport of purine derivatives into vacuoles isolated from Saccharomyces cerevisiae was studied. Vacuoles which conserved their ability to take up purine compounds were prepared by a modification of the method of polybase-induced lysis of spheroplasts. Guanosine greater than inosine = hypoxanthine greater than adenosine were taken up with decreasing initial velocities, respectively; adenine was not transported. Guanosine and adenosine transporting systems were saturable, with apparent Km values 0.63 mM and 0.15 mM respectively, while uptake rates of inosine and of hypoxanthine were linear functions of their concentrations. Adenosine transport in vacuoles appeared strongly dependent on the growth phase of the cell culture. The system transporting adenosine was further characterized by its pH dependency optimum of 7.1 and its sensitivity to inhibition by S-adenosyl-L-methionine. In the absence of adenosine in the external medium, [14C]adenosine did not flow out from preloaded vacuoles. However, in the presence of external adenosine, a very rapid efflux of radioactivity was observed, indicating an exchange mechanism for the observed adenosine transport in the vacuoles. In isolated vacuoles the only purine derivative accumulat...Continue Reading

References

Feb 7, 1967·Biochimica Et Biophysica Acta·J E Cummins, J M Mitchison
Apr 4, 1967·Biochimica Et Biophysica Acta·O Stein, Y Stein
Aug 1, 1957·Biochimica Et Biophysica Acta·D B COWIE, E T BOLTON

Citations

Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Wenying ShouJose M G Vilar
Nov 20, 1981·Biochimica Et Biophysica Acta·B J NieuwenhuisG W Borst-Pauwels
Nov 13, 2008·Molecular & Cellular Proteomics : MCP·Elena WiederholdDirk J Slotboom
Aug 15, 1997·The Journal of Biological Chemistry·J W Booth, G Guidotti

Related Concepts

Adenocard
Cell Division Phases
Guanosine
Hydrogen-Ion Concentration
Hypoxanthines
Inosine
Organoids
Purines
Saccharomyces cerevisiae
Spheroplasts

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