PMID: 78Dec 5, 1975

Studies on rat renal cortical cell kallikrein. I. Separation and measurement

Biochimica Et Biophysica Acta
T Kaizu, H S Margolius


A technique has been developed to separate and measure kallikrein in a heterogeneous population of rat renal cortical cells in suspension. After rat kidneys were perfused in situ in anaesthetized rats, viable, counted cortical cell suspensions were obtained. Cells were suspended in a sucrose/Tris buffer containing 0.5% deoxycholate, homogenized, centrifuged, dialyzed, and gel filtered on Sephadex G-25. Column chromatography on DEAE-cellulose resulted in a single peak of esterase activity between 0.20 to 0.25 M NaCl/sodium phosphate buffer. Subsequent elution yielded an alkaline esterase which was identical to kallikrein isolated from rat urine, insofar as pH optimum, effects of inhibitors, bioassay activity and immunological properties were concerned. Calculated yields were about 70% of the total esterase activity present in the parent cell homogenates. Recoveries of a purified rat urinary kallikrein added to the cell homogenates, the DEAE-cellulose columns, or the eluates from the columns ranged from 83-108% (mean 96%). Using this technique, it was found that the amount of kallikrein activity present in non-incubated renal cortical cells ranged from 0.6-10(-2) to 4.6 - 10(-2) alpha-N-tosyl-L-arginine methyl ester (Tos-Arg-OMe)...Continue Reading


Jan 1, 1981·Pflügers Archiv : European journal of physiology·K TomitaF Sakai
Jan 1, 1978·General Pharmacology·K NustadJ V Pierce
Jun 1, 1980·The Journal of Clinical Investigation·H S Margolius, J Chao
Nov 1, 2011·Clinical and Experimental Nephrology·Lawrence G PalmerGustavo Frindt
Jan 30, 1978·Archives of Biochemistry and Biophysics·J Chao
Dec 15, 1982·Biochemical and Biophysical Research Communications·M TakaokaS Morimoto
Jul 1, 1979·Biochemical Pharmacology·J Chao, H S Margolius
Dec 29, 2000·Japanese Journal of Pharmacology·T FujitaM Majima
Mar 1, 1987·Kidney International·M WeinbergR J Solomon
Nov 1, 1981·Clinics in Endocrinology and Metabolism·K Abe
Apr 1, 1979·Circulation Research·N G Levinsky
Sep 1, 1981·Acta Pharmacologica Et Toxicologica·U B Olsen, E Eilertsen
Mar 17, 2021·AACN Advanced Critical Care·Maureen Scarboro, Karen A McQuillan
Sep 1, 1992·The American Journal of Physiology·S AvigdorJ Bouhnik

Related Concepts

Aldosterone, (11 beta,17 alpha)-Isomer
Immunodiffusion Measurement
Hydrogen-Ion Concentration
Structure of Cortex of Kidney
Tissue Specificity
Spiro von ct

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.