PMID: 698232Aug 23, 1978Paper

Studies on sheep kidney nuclease. II. Limited digestion of phiX174 single-strand DNA

Biochimica Et Biophysica Acta
T Watanabe

Abstract

Single strand DNA of bacteriophage phiX174 was digested by sheep kidney nuclease (nuclease SK) at 37 degrees C for 2-6 h and the digests were examined by 2.5% polyacrylamide gel electrophoresis. On this electrophoresis five bands were detected corresponding to the DNA fragments by scanning the gel at 260 nm. After these DNA fragments from the gel with the electrode buffer used for the electrophoresis, the molecular weights of fragments I, II, III, IV and V were determined by sucrose density gradient centrifugation. The molecular weights of these DNA fragments were 1.7 . 10(6), 1.2 . 10(6), 8.6 . 10(5), 4.5 . 10(5) and 2.8 . 10(5). From the melting curves and the base compositions of these fragments it was suggested that phiX174 single-strand DNA might have intramolecular double helical regions, which were hardly susceptible to attack by nuclease SK.

References

Aug 23, 1978·Biochimica Et Biophysica Acta·T Watanabe, K Kasai
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J F Morrow, P Berg
Jul 28, 1970·Journal of Molecular Biology·H O Smith, K W Wilcox
Jul 28, 1970·Journal of Molecular Biology·T J Kelly, H O Smith
Jun 28, 1967·Journal of Molecular Biology·D H BishopS Spiegelman
Aug 12, 1969·Biochimica Et Biophysica Acta·R Chandrasekaran, R Balasubramanian
Jan 1, 1954·Methods of Biochemical Analysis·E VOLKIN, W E COHN
Jul 1, 1963·Biophysical Journal·E BURGI, A D HERSHEY

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Citations

Jan 1, 1995·Critical Reviews in Microbiology·S U Gite, V Shankar

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