PMID: 6964320Oct 20, 1982Paper

Studies on steroidogenesis in the oocyte

Nihon Naibunpi Gakkai zasshi
O TsutsumiS Sakamoto

Abstract

The steroid hormone has an important role in the early stages of reproduction. There has been abundant histochemical evidence that oocytes contain steroid hormones and are able to synthesize these hormones. But there have been few methods of analyzing one oocyte biochemically because it is too small and light. In order to study steroidogenesis in the oocyte, a microassay method sensitive enough to analyze the enzyme activities in one oocyte was developed using enzymatic cyling for amplifying the reaction product to 10,000-fold. An oil-well technique and a microtube method were applied in the assay for achieving the reaction in a medium as small as 1.0 to 5.0 microliters under a stereomicroscope. Immature Wistar rats were superovulated by PMS-hCG administration. Oocytes were collected by puncturing the follicle and flushing the tube. They were freeze-dried after washing to remove cumulus cells. The dry weight of one oocyte was 51.2 +/- 6.2 ng in a quartz fiber fishpole balance. The activity of 3 beta hydroxysteroid dehydrogenase (3 beta HSD) (picomol/oocyte/hr, substrate:pregnenolone) in the PMS-treated oocyte was 2.66 +/- 0.59, which corresponds to 3 times the activity of the ovarian homogenate as control, indicating the high c...Continue Reading

Citations

Jan 8, 2011·Cell Biochemistry and Function·Sritulasi Karri, Vanithakumari G
Oct 19, 2006·Animal Reproduction Science·A Shirazi, Z Moalemian
Feb 18, 2004·The Journal of Reproduction and Development·Sueo Niimura, Shin-ya Kawakami

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