PMID: 41613Dec 14, 1979

Studies on synaptic vesicles in mammalian brain characterization of highly purified synaptic vesicles from bovine cerebral cortex

Brain Research
N Zisapel, N Zurgil

Abstract

Synaptic vesicles have been isolated from bovine cerebral cortex by sequential differential and density gradient centrifugations followed by chromatography on a Sepharose 6B column. We have studied the morphology, enzymatic markers, neurotransmitter and ATP contents and protein composition of the vesicles. The specific contents of acetylcholine, gamma-aminobutyric acid, aspartate, glutamate and catecholamines were 4--8-fold higher in the vesicle fraction compared to the crude synaptosomal pellet. Electron micrographs of the vesicle preparation showed enrichment of vesicular material with an average diameter of 50 nm. The purity of the preparation was assessed by the very low activities of enzymatic markers of cellular membranes and cytosol components. Some Ca--Mg-activated ATPase activity was detected in the vesicle preparations, but its content relative to the neurotransmitters fell on chromatography, suggesting that this activity may be partially contributed by non-synaptic vesicle components, such as small microsomes. The isolated synaptic vesicles were solubilized with 1% sodium dodecyl sulfate and subjected to polyacrylamide gel electrophoresis. The major Coomassie blue stained bands observed with apparent molecular weight...Continue Reading

References

Nov 1, 1975·Analytical Biochemistry·G A KimmichJ S Brand
Jun 11, 1976·Brain Research·A NagyV P Whittaker
Jan 1, 1977·Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry·P Lähdesmäki, R Winter
Jan 1, 1977·Journal of Neuroscience Research·C Richter-LandsbergT V Waehneldt
Jul 1, 1973·Journal of Neurochemistry·J T Coyle, D Henry
Feb 2, 1973·Science·S BerlW J Nicklas
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A L Blitz, R E Fine
Aug 1, 1972·The Journal of Physiology·B Katz, R Miledi
Feb 1, 1964·The Biochemical Journal·V P WhittakerR J Kirkland
Feb 1, 1957·Archives of Biochemistry and Biophysics·M H APRISON, P NATHAN
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE

Citations

Apr 28, 2006·Pflügers Archiv : European journal of physiology·Yuri PankratovR Alan North
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R JahnP Greengard
Sep 5, 2002·Nature Reviews. Neuroscience·Maiken NedergaardAnker J Hansen
May 1, 1984·Journal of Neurochemistry·F A AnthonyS K Sharma
Jun 1, 1985·Archives of Biochemistry and Biophysics·W Demmer, K Brand
Oct 22, 1983·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·P R Gordon-Weeks, D H Jones
Jan 1, 1980·Journal of Neurochemistry·N ZisapelI Gozes
Sep 21, 2013·Neuroreport·Francesca CortiFelicita Pedata

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Acetylthiocholinesterase
Ca(2+)-Transporting ATPase
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Adenosine Triphosphate, Chromium Ammonium Salt
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Cell Fractionation
Insula of Reil
Molecular Sieve Chromatography
Cytochrome c Oxidase Subunit VIa
Lactate Dehydrogenase

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