PMID: 590658Oct 13, 1977

Studies on the action of the nuclear factor promoting actinomycin D-binding capacity of chromatin

Differentiation; Research in Biological Diversity
G D TumanishviliD V Dzidziguri


It is well known that actinomycin D binds to C-G pairs of DNA. The amount of actinomycin D bound to chromatin thus depends directly on the demasked sites of chromatin DNA. The actinomycin D binding of rat liver chromatin, obtained by the method of Dingman and Sporn, was studied in the presence and absence of liver and kidney nuclear extracts (NE). The actinomycin D binding of liver chromatin increases greatly under the action of liver nuclear extract. No changes occur in liver chromatin actinomycin D binding capacity after the action of kidney NE. The removal of protein or RNA from liver NE removes its ability to change the actinomycin D binding capacity of the liver chromatin. According to the obtained results it may be assumed that the nuclear extract contains the factor which plays a role in controlling cell differentiation.


Mar 15, 1974·Experimental Cell Research·K M JandieriN D Mgvdeladze
Jan 21, 1969·Biochimica Et Biophysica Acta·N R Ringertz, L Bolund
Feb 14, 1971·Journal of Molecular Biology·L Kleiman, R C Huang
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·A E Mirsky

Related Concepts

Cell Differentiation Process
Cell Nucleus
Alkaline DNase
DNA, Double-Stranded
Tissue Specificity
Alkaline Ribonuclease

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