Jul 15, 1976

Studies on the amino acid-incorporating activity of native rat liver rough membrane and that reconstituted in vitro

The Biochemical Journal
N De GrootA A Hochberg

Abstract

The amino acid-incorporating activities of free polyribosomes, rough membranes and rough membranes reconstituted in vitro, derived from rat liver, were compared. The amino acid-incorporating activity of the two membrane fractions were very similar in their response towards changes in pH, Mg2+ concentration and temperature, but differed from the response of the amino acid-incorporating activity of free polyribosomes. Free polyribosomes irreversibly lost part of their amino acid-incorporating capacity after they had become bound to rough membrane, from which the original ribosomes had been removed. Ribonuclease activity present in the membrane fraction may be responsible for this loss.

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Mentioned in this Paper

Polyribosomes
Gene Products, Protein
Alkaline Ribonuclease
Endorphenyl
Leucine
Hydrogen-Ion Concentration
Metazoa
Liver
Magnesium
Rats, Laboratory

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