PMID: 6166721Jul 1, 1981Paper

Studies on the antigenic determinants in the self-association of IgG rheumatoid factor

The Journal of Experimental Medicine
F A NardellaM Mannik

Abstract

The number, location, and other characteristics of the antigenic determinants for self-association of IgG-rheumatoid factors (IgG-RF) were examined using the IgG-RF isolated from the plasma of one patient as a model system. Affinity chromatography was employed for isolation of the IgG-RF. Sedimentation equilibrium ultracentrifugation was used to study the various interactions. The antigenic valence of IgG-RF Fc, normal human Fc, and rabbit Fc fragments was two for the interaction with Fab fragments from IgG-RF, as might be expected from the molecular symmetry of IgG. The antigenic valence of intact normal IgG, however, was only one, indicating that when one of the available antigenic determinants interacted with the Fab fragment of IgG-RF, the other determinant becomes sterically inaccessible. Reduction and alkylation, known to increase the flexibility of the hinge region, did not alter the antigenic valence of IgG for Fab fragments of IgG-RF. The antigenic valence of IgG-RF in self-association could not be experimentally determined but must be two to permit the observed concentration-dependent further polymer formation of IgG-RF dimers. Unique antigenic determinants on the Fc fragments of IgG-RF were sought and not found, thus...Continue Reading

References

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Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D G RomansK J Dorrington
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R M PopeM Mannik
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Citations

Jan 1, 1988·Springer Seminars in Immunopathology·M MannikE H Sasso
Jan 1, 1990·Agents and Actions·P Christensen, A K Schröder
Jul 6, 1984·Journal of Immunological Methods·I Teitsson, H Valdimarsson
Nov 22, 1991·Journal of Immunological Methods·M Mannik, R E Person
Apr 8, 1992·Journal of Immunological Methods·M M Newkirk
Mar 1, 1993·Molecular Immunology·R C WilliamsA Solomon
Jul 20, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·S KimuraK Gomi
Feb 12, 1998·The New England Journal of Medicine·W V Epstein
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S E ArtandiV R Bonagura
Dec 1, 1985·The Journal of Experimental Medicine·F A NardellaM Mannik
Sep 1, 1987·The Journal of Experimental Medicine·I R OppligerM Mannik
Jul 1, 1992·Scandinavian Journal of Immunology·H G OttenF C Breedveld
Oct 1, 1994·Scandinavian Journal of Immunology·R C Williams, C C Malone
Mar 1, 1992·The Journal of Clinical Investigation·J S AxfordI M Roitt
Jan 1, 1988·Scandinavian Journal of Rheumatology. Supplement·F Milgrom
Jan 1, 1988·Scandinavian Journal of Rheumatology. Supplement·R E Schrohenloher
Jan 1, 1988·Scandinavian Journal of Rheumatology. Supplement·A K Schröder, P Christensen
Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·J GergelyE Rajnavölgyi
Mar 30, 2010·Journal of Immunological Methods·Suzanna TatarewiczMichael S Moxness
Sep 12, 2007·Journal of Clinical Laboratory Analysis·Mariana Tellez AraizaErasmo Martínez-Cordero
Jan 31, 2009·European Journal of Immunology·Phillip R KramerJayne Reuben
Oct 1, 1984·Arthritis and Rheumatism·F A NardellaM Mannik
Sep 1, 1982·Arthritis and Rheumatism·P B BrownM Mannik
Sep 13, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Guoqiang GuanPhillip R Kramer
Jan 1, 1988·Springer Seminars in Immunopathology·T W RademacherI Roitt

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