PMID: 13563755Aug 1, 1958Paper

Studies on the antigenicity of beta- and alpha-lipoproteins of human serum

The Journal of Experimental Medicine
A SCANUI H PAGE

Abstract

The techniques of agar immunoelectrophoresis and agar double diffusion were applied to the study of the antigenicity of beta- and alpha(1)-lipoproteins separated by ultracentrifugation from normal human sera. The effects of delipidation were also investigated. It was shown that beta- and alpha(1)-lipoproteins are antigenically distinct. For each class of lipoprotein studied, a single antigenic component was demonstrated. In some, but not all, preparations of alpha(1)-lipoprotein a second, small antigenic component was detected, and identified as albumin. Absorption with lipoprotein-free serum or albumin removed this component without changing the lipoprotein band. Delipidation did not affect the antigenicity of either beta- or alpha(1)-lipoproteins. Immunoelectrophoresis, because of its high sensitivity and specificity, provides an additional criterion of purity for antigenic proteins in addition to the data that can be obtained from ultracentrifugal and free electrophoretic analysis.

References

Feb 4, 1955·Science·L KORNGOLD, R LIPARI
Jan 1, 1957·The Journal of Experimental Medicine·F ALADJEMM LIEBERMAN
Jan 26, 1957·Nature·F ALADJEM, D H CAMPBELL
Aug 1, 1957·The Journal of Experimental Medicine·L DELALLAR K BROWN
Apr 1, 1958·Archives of Biochemistry and Biophysics·A SCANUF M BUMPUS

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Citations

Dec 1, 1964·Immunochemistry·K W WALTON, S J DARKE
Mar 1, 1959·The Journal of Experimental Medicine·A SCANU, I H PAGE
Jul 1, 1959·The Journal of Experimental Medicine·W W BRINERD G CORNWELL
Apr 1, 1961·The Journal of Experimental Medicine·A SCANU, P ORIENTE
May 1, 1963·Vox Sanguinis·W GREUTER, R BUTLER
Jan 1, 1973·Journal of Clinical Pathology. Supplement (Association of Clinical Pathologists)·D G Cramp
Aug 1, 1962·The Journal of Clinical Investigation·A SCANU, W L HUGHES
Feb 1, 1964·The Journal of Clinical Investigation·D S FREDRICKSON
Mar 1, 1965·The Journal of Clinical Investigation·R I LEVY, D S FREDRICKSON
Jan 1, 1966·The Journal of Clinical Investigation·R I LevyD S Fredrickson
Apr 1, 1966·The Journal of Clinical Investigation·R I LevyL Laster
Feb 1, 1970·The Journal of Clinical Investigation·D RudmanC H Howard
Feb 1, 1966·The American Journal of Medicine·L CohenL Dobrilovic
Apr 19, 1968·Biochemical and Biophysical Research Communications·A M GottoD S Fredrickson
Jun 15, 1959·Klinische Wochenschrift·E FRICK
Aug 28, 1959·Annals of the New York Academy of Sciences·W A BARDAWIL, B L TOY
Apr 20, 1964·Biochimica Et Biophysica Acta·A GUSTAFSON
Jan 1, 1982·La Ricerca in Clinica E in Laboratorio·P Alaupovic

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