Abstract
The variant surface glycoprotein (VSG) of several Trypanosoma brucei clones has been metabolically labeled with [35S]methionine in parasites grown in the presence or in the absence of tunicamycin. Pulse and chase experiments followed by immunoprecipiation with anti-VSG sera and polyacrylamide gel electrophoresis of the immunoprecipitates, have helped to elucidate two steps of the sequence of glycosylation of VSG. Immediately after, or concomitantly with the synthesis of the protein, a first type of oligosaccharide side chain is also synthesized. Tunicamycin, a specific inhibitor of asparagine glycosylation, completely inhibits this reaction. The total amount of VSG found in trypanosomes grown in the presence of tunicamycin is less than in controls. The unglycosylated molecule has an apparent molecular weight 5-10% smaller than the mature form. A subsequent step, occurring after translation, is the synthesis of carbohydrate side chains which contain a cross-reacting antigenic determinant detected in all VSGs so far studied by immunoprecipitations with heterologous antisera. The percentage of total VSG bearing sugars involved in cross-reactions is variable in different clones, increases with time and subsequently decreases, sugge...Continue Reading
References
Dec 1, 1975·Parasitology·G A Cross
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A F Barbet, T C McGuire
Jan 22, 1979·Nature·G A Cross
Jul 1, 1979·Journal of General Microbiology·G A Cross
Apr 23, 1979·Biochimica Et Biophysica Acta·A J Parodi, L F Leloir
Sep 4, 1979·Biochemistry·R K KellerF C Crum
Nov 1, 1977·The Journal of protozoology·J G Johnson, G A Cross
Feb 16, 1978·Nature·L RovisR O Williams
Mar 1, 1978·Cell·K OldenK M Yamada
Sep 1, 1978·Nucleic Acids Research·R O WilliamsS C Williams
Jul 2, 1973·European Journal of Biochemistry·N J Cowan, C Milstein
Dec 1, 1973·Parasitology·G A Cross, J C Manning
Dec 1, 1970·Experimental Parasitology·S M Lanham, D G Godfrey
Apr 1, 1972·Endocrinology·D Gospodarowicz
Nov 1, 1971·The Journal of Antibiotics·A Takatsuki, G Tamura
Dec 13, 1969·Nature·K Vickerman, A G Luckins
Oct 1, 1980·Experimental Parasitology·T C McGuireJ J Doyle
Feb 1, 1981·Molecular and Biochemical Parasitology·A A Holder, G A Cross
Jun 1, 1980·Parasitology·L Rovis, S Baekkeskov
Aug 1, 1980·European Journal of Biochemistry·C T Brett, H P Voorheis
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J E Strickler, C L Patton
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J M Prives, K Olden
Oct 1, 1982·Thrombosis Research·K NibuN Hamasaki
Mar 1, 1980·Gene·J H HoeijmakersG A Cross
Dec 11, 1980·Nature·J C BoothroydP Borst
Citations
Jan 1, 1984·Pharmacology & Therapeutics·S R Meshnick
Mar 1, 1982·Molecular and Biochemical Parasitology·L Rovis, S Dube
Sep 1, 1982·Molecular and Biochemical Parasitology·J McConnellM J Turner
Jun 1, 1983·Molecular and Biochemical Parasitology·J McConnellL Rovis
May 1, 1983·Molecular and Biochemical Parasitology·G DuvillierP Degand
Nov 1, 1983·Molecular and Biochemical Parasitology·M MilhausenN Agabian
Nov 1, 1984·Molecular and Biochemical Parasitology·E N MillerM J Turner
Jun 1, 1985·Molecular and Biochemical Parasitology·B ZingalesW Colli
Jun 1, 1985·Molecular and Biochemical Parasitology·P A MajiwaR Hamers
Jan 2, 1987·Molecular and Biochemical Parasitology·J K Lovelace, M Gottlieb
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D J GrabY Verjee
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J D BangsP T Englund
Jul 1, 1986·The Journal of Cell Biology·J D BangsP T Englund
Aug 1, 1984·The Journal of Cell Biology·D J GrabL Rovis
Sep 2, 1985·European Journal of Biochemistry·E Reinwald
Dec 1, 1982·Antimicrobial Agents and Chemotherapy·R A CaseroR J Bernacki
Jun 1, 1991·Acta Tropica·E I Kamanga-SolloR A Masake
Feb 1, 1987·Experimental Parasitology·T O Frommel, A E Balber
Apr 1, 1986·Archives of Biochemistry and Biophysics·K A Presper, E C Heath
Jan 29, 1985·Biochimica Et Biophysica Acta·A Bernards