PMID: 1195339Apr 23, 1975Paper

Studies on the brush border membrane of the mouse duodenum. I. Membrane isolation and analysis of protein components

The Journal of Membrane Biology
T Billington, P R Nayudu

Abstract

Brush border membranes have been isolated from villus epithelial cells of the adult Swiss mouse duodenum. Preparations of these membranes are not contaminated by other organelles as judged from electron-micrographs of sectioned pellets of brush borders. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins from brush borders solubilized in Tris-sodium dodecyl sulfate buffer reveals a reproducible Coomassie Brilliant Blue pattern of 17 bands. By comparing the brush border protein band positions with those of standard proteins run concurrently on sodium dodecyl sulfate-polyacrylamide gel slabs it is estimated that the 17 brush border proteins and subunits have molecular weights ranging from over 250,000 to around 16,000. Periodate-fuchsin sulfite staining shows that the five more slowly migrating, high molecular weight proteins are glycoproteins. The two proteins of smallest molecular size react positively with Oil Red O but have very small amounts of lipophilic amino acid residues, which indicates that the lipid extractable from the gels in these areas is a contaminant and is not bound to the proteins.

References

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Oct 1, 1972·The Journal of Clinical Investigation·D H Alpers
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Oct 1, 1958·Proceedings of the Society for Experimental Biology and Medicine·J H PERT, H KUTT
May 25, 1959·The Journal of Biophysical and Biochemical Cytology·S L PALAY, L J KARLIN
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Citations

Jun 9, 1976·The Journal of Membrane Biology·T Billington, P R Nayudu
Jan 1, 1979·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M DaucaD Menard
Jan 15, 1978·FEBS Letters·B KaurC A Pasternak
Sep 30, 1976·Molecular and Cellular Biochemistry·R I SalganikV I Deribas
Jun 1, 1979·Journal of Human Nutrition·E F Wheeler
Jun 9, 1976·The Journal of Membrane Biology·T Billington, P R Nayudu

Related Concepts

Plasma Membrane
Duodenum
Epithelial Cells
Mesothelium
Structure of Intestinal Gland
Lipids
Electron Microscopy
Microscopy, Differential Interference Contrast

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