PMID: 1202018Dec 1, 1975Paper

Studies on the distribution of antigenic sites on the surface of rabbit spermatozoa

The Journal of Cell Biology
J K Koehler

Abstract

Antibodies to rabbit spermatozoa isolated from immunized virgin female rabbits were conjugated to hemocyanin and have been used to determine the pattern of antigenic sites over the surface of rabbit spermatozoa under a variety of conditions. When the spermatozoa are incubated with antibody at 0 degrees C, labeling is almost entirely restricted to the postacrosomal sheath. Incubation at room temperature (22 degrees C) or at 37 degrees C results in progressively increased acrosomal labeling as well as enhanced postacrosomal sheath labeling. Initial incubation at low temperature followed by washing and high temperature incubation yields cells which indicate little tendency for postacrosomal sheath sites to migrate toward the acrosomal region. Absorption of the antibody preparation with rat lymphocytes has no effect on labeling density, whereas absorption with rabbit lymphocytes diminishes the intensity of labeling and yields cells with markers primarily over the postacrosomal sheath. It is concluded that the postacrosomal sheath plasma membrane has specific antigenic properties distinct from other regions of the head.

References

Mar 1, 1975·Experimental Cell Research·R P EricksonD Tennenbaum
Dec 1, 1974·The Journal of Cell Biology·A Elgsaeter, D Branton
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M Edidin, A Weiss
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·G C KooM P Lardis
Dec 1, 1974·Developmental Biology·M FellousF Jacob
Dec 1, 1972·The American Journal of Anatomy·R YanagimachiG L Nicolson
Mar 1, 1974·The Journal of Cell Biology·J K Koehler, W D Perkins
May 1, 1974·Journal of Reproduction and Fertility·I Russo, C B Metz
Dec 1, 1970·Journal of Ultrastructure Research·J K Koehler
Jun 1, 1970·Biology of Reproduction·J M Bedford
Mar 1, 1972·Developmental Biology·S B Smith, J P Revel
Oct 1, 1972·The Journal of Experimental Medicine·M J KarnovskyM Leventhal

❮ Previous
Next ❯

Citations

Mar 1, 1978·Developmental Biology·B BaccettiA G Burrini
Oct 1, 1980·Developmental Biology·C F Millette, A R Bellvé
Aug 1, 1983·Developmental Biology·P Primakoff, D G Myles
Feb 1, 1981·International Journal of Andrology·M Vierula, H Rajaniemi
Jan 1, 1987·Archives of Andrology·S PilikianJ Lornage

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
G C KooM P Lardis
The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
P P Le BouteillerG A Voisin
© 2022 Meta ULC. All rights reserved