PMID: 6161293Jan 1, 1980Paper

Studies on the E. coli groNB (nusB) gene which affects bacteriophage lambda N gene function

Molecular & General Genetics : MGG
C P GeorgopoulosH Eisen

Abstract

Escherichia coli mutants, called groNB, which block the growth of bacteriophage lambda at the level of action of the gene N product, have been isolated as survivors at 42 degrees C of bacteria carrying a) the defective prophage lambda bio11 i lambda cI857 delta H1 or b) the pcR1 plasmid containing the EcoRI immunity fragment of phage lambda cI857. In addition, groNB bacterial mutants have been isolated at 37 degrees C, as large colony formers in the presence of lambda i lambda cI h434, lambda i lambda cI h lambda, and lambda i lambda cI h80 phage. The groNB locus is located at 9 minute of the E. coli genetic map with the order of the neighboring loci being proC tsx groNB purE. Most groNB mutations isolated at 42 degrees C were found to interfere in addition with bacterial growth at low temperatures, since (a) the GroNB phenotypes of lambda growth inhibition and bacterial cold sensitivity cannot be separated by P1 transduction, and (b) some cold resistant revertants simultaneously become Gro+ for lambda growth. Lambda transducing phages carrying the groNB+ bacterial gene have been isolated. GroNB mutant bacteria lysogenized by the transducing phage acquire the Gro+ phenotype and simultaneously the cold resistant phenotype, sugge...Continue Reading

References

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Citations

Jan 1, 1981·Molecular & General Genetics : MGG·J SwindleC Georgopoulos
Jan 1, 1981·Molecular & General Genetics : MGG·M Strauch, D I Friedman
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·F Warren, A Das
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R A SharrockM Nomura
Jun 25, 1984·Nucleic Acids Research·J SwindleC Georgopoulos
Oct 1, 1992·Current Opinion in Genetics & Development·D I Friedman
Jul 25, 1983·Journal of Molecular Biology·D F WardM E Gottesman
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
May 28, 1982·Science·D F Ward, M E Gottesman

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