PMID: 2505760Jul 15, 1989Paper

Studies on the effect of lysosomotropic agents on the release of Gal beta 1-4GlcNAc alpha-2,6-sialytransferase from rat liver slices during the acute-phase response

The Biochemical Journal
G Lammers, J C Jamieson

Abstract

The mechanism of release of Gal beta 1-4GlcNAc alpha-2,6-sialyltransferase (CMP-N-acetylneuraminate: beta-galactoside alpha-2,6-sialytransferase, EC 2.4.99.1) from rat liver during the acute-phase response is due to the action of a cathepsin D-like proteinase that cleaves the trans-Golgi membrane-bound enzyme from a membrane anchor; this allows a major portion of the enzyme containing the catalytic site to escape into the extracellular space [Lammers & Jamieson (1988) Biochem. J. 256, 623-631]. The release of sialytransferase was most effective at pH 5.6, suggesting that release of sialyltransferase from the Golgi in whole cells is dependent on maintaining an acidic environment in the trans-Golgi compartment of the hepatocyte. Golgi membranes contain a proton pump that maintains the acidic pH in these compartments [Glickman, Croen, Kelly & Al-Awquati (1983) J. Cell Biol. 97, 1303-1308; Yamashiro, Tycko & Maxfield (1984) Cell (Cambridge, Mass.) 37, 789-800; Zhang & Schneider (1983) Biochem. Biophys. Res. Commun. 114, 620-625; Anderson & Pathak (1985) Cell (Cambridge, Mass.) 40, 635-643]. Lysosomotropic agents, such as NH4Cl, chloroquine and methylamine can penetrate acidic compartments of the cell, such as the Golgi complex, rai...Continue Reading

Citations

Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·G J Strous, J Dekker
May 23, 1997·The Journal of Biological Chemistry·S K Cho, R D Cummings
Apr 1, 2017·BMC Bioinformatics·Wei-Sheng TienKun-Pin Wu
Jan 1, 2021·Frontiers in Cardiovascular Medicine·Alkmini A Papadopoulou, Regina Fluhrer
Jan 10, 2002·Biochimica Et Biophysica Acta·Elena V GrachevaNina V Prokazova
May 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J C JamiesonP G Harder
Sep 21, 2013·ACS Chemical Biology·Rudo L SimeonZhilei Chen
May 1, 1993·Pathology, Research and Practice·W ReedJ M Nesland

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