PMID: 737167Dec 19, 1978Paper

Studies on the effect of the lipid phase transition on the interaction of glucagon with dimyristoyl glycerophosphocholine

Biochimica Et Biophysica Acta
R M Epand

Abstract

Glucagon is found to interact with dimyristoyl glycerophosphocholine both above and below the phase transition temperature of the lipid. Above the phase transition temperature the interaction is manifested by an increase in the rate of vesicle aggregation and by an increased permeability of unilamellar vesicles to Eu3+ and to Fe(CN)3-6. However, no stable lipoprotein complex can be detected by gel filtration. Below the phase transition glucagon can form stable complexes with dimyristoyl glycerophosphocholine vesicles but cannot rapidly rearrange these vesicles to disk-shaped particles until the phase transition temperature is approached. The energy of activation for the dissociation of glucagon from the disk-shaped lipoprotein particle is 29 kcal/mol at temperatures above 36 degrees C but increases markedly at lower temperatures, as the region of the lipid phase transition is approached. This increase in energy of activation at lower temperatures is most probably due to the larger amount of energy required to rearrange gel-state lipid in the transition state and provides an explanation for the unusual kinetic stability of the glucagon-dimyristoyl glycerophosphocholine lipoprotein complex only at temperatures below the phase tra...Continue Reading

References

Aug 9, 1977·Biochimica Et Biophysica Acta·J D MorrisettA M Gotto
Mar 28, 1977·Biochimica Et Biophysica Acta·R M EpandS Schreier
Jan 19, 1978·Biochimica Et Biophysica Acta·P W van DijckJ de Gier
May 2, 1977·Biochimica Et Biophysica Acta·H L KantorJ M Sturtevant
Jun 14, 1977·Biochemistry·Y BarenholzR D Carlson
Dec 24, 1970·Biochemical and Biophysical Research Communications·P Hinkle
Sep 27, 1967·Biochemical and Biophysical Research Communications·W B GratzerG H Beaven

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Citations

Jan 1, 1982·Biophysical Journal·R M Epand, J M Sturtevant
Nov 1, 1983·Archives of Biochemistry and Biophysics·H S HendricksonD E Kleiner
Aug 24, 1983·Biochimica Et Biophysica Acta·J R ErnandesS Schreier
Oct 7, 1997·Biochimica Et Biophysica Acta·I V PolozovR M Epand
Dec 21, 1981·Biochimica Et Biophysica Acta·R M EpandS W Hui
Jun 1, 1985·International Journal of Peptide and Protein Research·R M EpandM Rosenblatt
May 1, 1992·International Journal of Peptide and Protein Research·S KimuraR Schwyzer
Jul 17, 1998·Biochimica Et Biophysica Acta·R Koynova, M Caffrey
Apr 14, 1988·Biochimica Et Biophysica Acta·P PastaG Carrea

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