Studies on the energy metabolism of opossum (Didelphis Virginiana) erythrocytes. I. Utilization of carbohydrates and purine nucleosides

Journal of Cellular Physiology
N C BethlenfalvayT Waldrup

Abstract

Opossum erythrocytes filtered through cellulose columns were used to estimate their permeability to D-glucose and optimum inorganic phosphate requirement for D-glucose utilization at pH 7.4 and 8.1. D-Glucose readily penetrated opossum red cells; there was no measurable difference whether plasma or electrolyte solution served as the suspending medium. Optimum extracellular inorganic phosphate concentration for glucose utilization as indicated by red cell lactate production was pH-dependent, with a sharp optimum of 30 mmol/liter at pH 8.1. Whereas glucose, fructose, mannose, dihydroxyacetone, adenosine, and inosine were readily utilized at pH 7.4 and Pi 30 mmol/liter as shown by net lactate and ATP production by the red cells, galactose and ribose as substrates were not metabolized. In electrolyte, Pi 30 mmol/liter, and pH 7.4 glucose utilization by opossum red cells averaged 3.5 mumol, at pH 8.1, 9.5 mumol/ml cells/hr were utilized. Red cells suspended in leukocyte-free plasma utilized D-glucose at a rate of 3.0 mumol/ml/hr at pH 7.5. Seven percent of D-glucose flowed through the pentose phosphate pathway; this rate increased 11-fold by methylene blue stimulation. The amount of D-glucose recycled through the pentose phosphate p...Continue Reading

References

Dec 15, 1977·Experientia·N S Agar
Oct 1, 1976·Journal of Cellular Physiology·J W Harvey, J J Kaneko
Aug 1, 1972·The Australian Journal of Experimental Biology and Medical Science·J D GuptaJ D Harley
Jan 1, 1983·Comparative Biochemistry and Physiology. A, Comparative Physiology·N C BethlenfalvayT Waldrup
Jan 1, 1980·The Journal of Clinical Investigation·T Kondo, E Beutler
Jun 1, 1962·The Journal of Clinical Investigation·R G CHAPMANB W GABRIO
Jul 1, 1964·The Journal of Clinical Investigation·E R SIMON, P WAYS

Citations

Jan 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·N S Agar, I R Godwin
Dec 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·N S Agar, I R Godwin
Mar 1, 1995·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·A L ParkinsonN S Agar

Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Blood Glucose
Sugars
Cell Membrane Permeability
Chromelin
Energy Metabolism
Erythrocytes
Hexoses
Lactates
Didelphidae

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